biologia plantarum

Biologia plantarum 49:23-28, 2005 | DOI: 10.1007/s10535-005-3028-4

Microspore development during in vitro androgenesis in triticale

J. M. González^1,*, N. Jouve¹

¹ Departamento de Biología Celular y Genética, Facultad de Biología, Universidad de Alcalá, Campus Universitario, Alcalá de Henares (Madrid), Spain

Microspore division was monitored in three triticale (× Triticosecale Wittmack) genotypes over 21 d of in vitro anther culture, on two media differing in their 2,4-dichlorophenoxyacetic acid content. After low temperature (4 °C) pre-treatment for two weeks, all the microspores were still alive, but they began to die from day one of culture. Both genotype and culture medium affected the number of microspores that aborted over time (82 - 97 % by day 21), the number of microspores that underwent the first symmetrical division (> 82 % over all), the number of microspores that attained four or more nuclei, and the number of divisions per 100 alive microspores after 21 d of culture.

Keywords: anther culture; 2; 4-D; haploid; low temperature; × Triticosecale

Subjects: androgenesis, anther culture, androgenic embryos; cultivar and genotype differences, microspore development; in vitro culture, anther culture; microspore development, in vitro culture; nutrient medium N6 (Chu); ploidy; temperature, low, androgenesis; triticale, × Triticosecale, microspore development

Received: October 31, 2003; Accepted: April 27, 2004; Published: March 1, 2005  Show citation