biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:29-33, 2005 | DOI: 10.1007/s10535-005-0033-6

Direct somatic embryogenesis and plant regeneration from ray florets of chrysanthemum

A. K. A. Mandal1, S. K. Datta1,*
1 Floriculture Section, National Botanical Research Institute, Lucknow, India

Direct somatic embryogenesis from ray floret explants of five chrysanthemum cultivars has been obtained within 12 - 15 d on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzyladenine (BA). Scanning electron microscopic observation also confirmed the direct origin of somatic embryos from explants. Somatic embryos developed asynchronously on the adaxial surface of explants. Among the five cultivars tested, Birbal Sahani was best responding (40 % explants responded on 4 mg dm-3 2,4-D and 2 mg dm-3 BA supplemented medium). Precocious germination of somatic embryos was noticed on the same medium. The best sucrose concentration in the medium was found to be 60 g dm-3 where 70 % explants responded while 55 % embryogenic response was obtained on medium supplemented with 400 mg dm-3 inositol. Plants developed from somatic embryos were transferred to soil and produced true-to-type flowers.

Keywords: 6-benzyladenine; Chrysanthemum morifolium; cultivar differences; 2; 4-dichloro-phenoxyacetic acid; naphthaleneacetic acid
Subjects: auxins; Chrysanthemum morifolium; chrysanthemum, somatic embryogenesis; cultivar and genotype differences, somatic embryogenesis, regeneration from ray florets; cytokinins; embryo development, somatic; flower; in vitro culture, regeneration, organogenesis, morphogenesis; in vitro culture, somatic embryogenesis; in vitro culture, sugar sources; inositol, in vitro culture; nutrient medium MS (Murashige and Skoog); sucrose, in vitro culture

Received: September 18, 2003; Accepted: August 11, 2004; Published: March 1, 2005  Show citation

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Mandal, A.K.A., & Datta, S.K. (2005). Direct somatic embryogenesis and plant regeneration from ray florets of chrysanthemum. Biologia plantarum, 49(1), 29-33. doi: 10.1007/s10535-005-0033-6
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