biologia plantarum

Biologia plantarum 49:41-45, 2005 | DOI: 10.1007/s10535-005-1045-y

In vitro culture of Feronia limonia (L.) Swingle from hypocotyl and internodal explants

L. V. Hiregoudar¹, H. G. Ashok Kumar¹, H. N. Murthy^1,*

¹ Department of Botany, Karnatak University, Dharwad, India

The hypocotyl and internodal segments from in vitro grown seedlings of Feronia limonia (L.) Swingle (wood apple) were cultivated on Murashige and Skoog's (1962, MS) medium supplemented with N⁶-benzyladenine (BA) or adenine (ADE) or kinetin (KN) at 0.5 to 5 µM. The optimum response was recorded on the medium containing 2 µM BA. An average of 12 and 8 shoots were developed from hypocotyl and internodal explants, respectively, after eight weeks of culture. The shoots were excised, and the residual explants were transferred to fresh medium where again they developed shoots. Up to three such passages resulted in the production of shoots from repeatedly subcultured explants and an average of 24 - 36 shoots per explant was obtained. The in vitro developed shoots produced roots when transferred to half strength MS medium supplemented with 1 µM 1-naphthaleneacetic acid (NAA). The developed plantlets were successfully transferred to mixture of soil, sand and coco-peat (1:1:1) and hardened in controlled environment. Hardened plants were transplanted to soil in greenhouse.

Keywords: adventitious shoots; medicinal plant; micropropagation; organogenesis

Subjects: adventitious shoot, root; auxins; cytokinins; ex vitro transfer; Feronia limonia; in vitro culture, acclimation, adaptation, to ex vitro transfer; in vitro culture, adventitious shoots and roots; in vitro culture, from cotyledon; in vitro culture, from internodium; in vitro culture, micropropagation; in vitro culture, regeneration, organogenesis, morphogenesis; medicinal plants; nutrient medium MS (Murashige and Skoog); wood apple, adventitious shoots in vitro

Received: December 17, 2003; Accepted: August 26, 2004; Published: March 1, 2005  Show citation