biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:153-156, 2005 | DOI: 10.1007/s00000-005-3156-9

Water stress-induced oxidative damage and antioxidant responses in micropropagated banana plantlets

T. -T. Chai1,*, N. M. Fadzillah1, M. Kusnan1, M. Mahmood2
1 Department of Biology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, Serdang, Malaysia
2 Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, Universiti Putra Malaysia, Serdang, Malaysia

Oxidative injury and antioxidant responses were investigated in two banana genotypes (Musa AAA 'Berangan' and Musa AA 'Mas') subjected to 40 % PEG-induced water stress. PEG treatment resulted in oxidative injury, as expressed in increased lipid peroxidation and reduced membrane stability index, in both cultivars; however, greater oxidative injury was detected in 'Mas'. Under PEG treatment, catalase activity and glutathione reductase activity were enhanced in both cultivars, but were higher in 'Mas'. Ascorbate peroxidase activity was enhanced in 'Berangan' under water stress, but was unaffected in 'Mas'. Meanwhile, superoxide dismutase activity was inhibited in both cultivars under water stress, but higher activity was detected in 'Berangan'. Higher ascorbate peroxidase and superoxide dismutase activities were associated with greater protection against water stress-induced oxidative injury.

Keywords: oxidative stress; ascorbate peroxidase; superoxide dismutase; glutathione reductase; catalase; lipid peroxidation
Subjects: antioxidants, enzymes; antioxidants, micropropagated banana plantlets; ascorbate peroxidase; catalase; glutathione reductase; lipid peroxidation; malondialdehyde; membrane stability index (MSI); Musa AA 'Mas'; Musa AAA 'Berangan'; nutrient medium MS (Murashige and Skoog); oxidative stress, antioxidants; photon flux density; superoxide dismutase; water stress, oxidative damage, antioxidants

Received: February 2, 2004; Accepted: September 14, 2004; Published: March 1, 2005  Show citation

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Chai, T.-T., Fadzillah, N.M., Kusnan, M., & Mahmood, M. (2005). Water stress-induced oxidative damage and antioxidant responses in micropropagated banana plantlets. Biologia plantarum, 49(1), 153-156. doi: 10.1007/s00000-005-3156-9
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