biologia plantarum

Biologia plantarum 49:257-260, 2005 | DOI: 10.1007/s10535-005-7260-8

An efficient in vitro method for mass propagation of Tylophora indica

M. Faisal¹, M. Anis^1,*

¹ Plant Tissue Culture Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, India

A protocol of high frequency shoot organogenesis and plant establishment from stem derived callus has been developed for Tylophora indica (Burm. f.) Merrill. - an endangered medicinal plant. Callus was developed on Murashige and Skoog (MS) medium supplemented with 10 μM 2,4,5-trichlorophenoxy acetic acid (2,4,5-T). Multiple shoot induction was achieved from the surface of the callus after transferring onto shoot induction medium. The highest rate (80 %) of shoot multiplication was achieved on MS medium containing 5.0 μM kinetin. The developed shoots rooted best on half-strength MS medium supplemented with 0.5 μM indole-3-butyric acid (IBA). The in vitro raised plantlets with well developed shoot and roots were acclimatized successfully and grown in greenhouse.

Keywords: callus culture; growth regulators; medicinal plant; shoot multiplication; 2,4,5-trichlorophenoxy acetic acid

Subjects: auxins; cytokinins; in vitro culture, callus induction; in vitro culture, mass propagation; in vitro culture, regeneration, organogenesis, morphogenesis; medicinal plants; nutrient medium MS (Murashige and Skoog); Tyloprora indica

Received: June 10, 2003; Accepted: October 18, 2004; Published: June 1, 2005  Show citation