biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:261-264, 2005 | DOI: 10.1007/s10535-005-1264-2

The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba

D. Šafářová1,*, D. Kopecký2, J. Vagera3
1 Department of Cell Biology and Genetics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, Olomouc-Holice, Czech Republic
2 Department of Botany, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, Olomouc-Holice, Czech Republic
3 Institute of Experimental Botany, Academy of Sciences of Czech Republic, Sokolovská 6, Olomouc, Czech Republic

The effect of a short heat treatment in combination with different culture medium composition on the efficiency of in vitro induced androgenesis in Silene latifolia ssp. alba was studied. The heat shocks (33 and 37°C) were applied for 1, 3, and 5 d. The best androgenic response was observed at 25°C and after a one-day treatment at 33°C. All other treatments reduced androgenic response. Among different media compositions tested, the most satisfactory results were obtained on BMS medium supplemented with 6-benzylaminopurine (0.5 mg dm-3) and sucrose. The green, albino and chimeric, only female, plants were regenerated. Flow cytometry of 110 regenerants identified haploids, mixoploids (n+2n and 2n+4n) and dihaploids.

Keywords: campion; green regenerants; ploidy analysis
Subjects: androgenesis, anther culture, androgenic embryos; auxins; campion, induced androgenesis; cytokinins; heat treatment, short, androgenesis; nuclear DNA content; ploidy; Silene latifolia ssp. alba

Received: October 17, 2003; Accepted: August 11, 2004; Published: June 1, 2005  Show citation

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Šafářová, D., Kopecký, D., & Vagera, J. (2005). The effect of a short heat treatment on the in vitro induced androgenesis in Silene latifolia ssp. alba. Biologia plantarum, 49(2), 261-264. doi: 10.1007/s10535-005-1264-2
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