biologia plantarum

Biologia plantarum 49:281-284, 2005 | DOI: 10.1007/s10535-005-1284-y

In vitro regeneration of mature Pinus sylvestris buds stored at freezing temperatures

U. Andersone¹, G. Ievinsh^1,*

¹ National Botanic Garden, Salaspils, Latvia

Changes of morphogenic competence in mature P. sylvestris L. buds due to frozen storage were investigated. The highest callus formation was registered on explants stored at -18°C for three months, but on explants stored for five months, it was also higher than in the control. Budding and development of needles in vitro was observed only for buds frozen three to five months. Peroxidase activity was lowest in these buds. In contrast, polyphenol oxidase activity in bud tissues continually increased during frozen storage. Within 10 months of frozen storage the content of starch and sugars in resting buds changed. It may be concluded that changes in composition of non-structural sugars in pine buds after five months of frozen storage are part of metabolic changes leading to loss of morphogenic capacity.

Keywords: peroxidase; polyphenol oxidase; Scots pine; sugars

Subjects: bud; frozen storage of mature buds in vitro; in vitro culture, axillary buds proliferation, bud breaking and regeneration; peroxidase; Pinus sylvestris; polyphenol oxidase; Scots pine, in vitro culture, regeneration of buds; starch, frozen storage of mature buds, flower, seed development; sugars, starch, frozen storage of mature buds

Received: April 5, 2004; Accepted: October 1, 2004; Published: June 1, 2005  Show citation