biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:293-296, 2005 | DOI: 10.1007/s10535-005-3296-z

Effect of arsenic on some physiological parameters in bean plants

N. Stoeva1,*, M. Berova1, Z. Zlatev1
1 Department of Plant Physiology and Biochemistry, Agricultural University of Plovdiv, Plovdiv, Bulgaria

The objective of the study was to investigate the effect of different arsenic concentrations on some physiological parameters of bean (Phaseolus vulgaris L.) cultivars Plovdiv 10 and Prelom in the early growth phases. Seedlings, grown in sand with Hoagland-Arnon nutrient solution in a climatic box, were treated with 0, 2, 5 mg(As) dm-3 as Na3AsO4 (pH 5.5). After 5 d of As treatment, the changes in leaf gas-exchange, water potential, chlorophyll and protein contents, peroxidase activity and lipid peroxidation in roots were recorded. Physiological analysis showed a minor negative effect of arsenic at concentration 2 mg(As) dm-3, but at the higher dosage of 5 mg(As) dm-3 growth, leaf gas-exchange, water potential, protein content and biomass accumulation were reduced in both cultivars. The peroxidase activity and lipid peroxidation increased considerably at 5 mg(As) dm-3, which is a typical reaction of the plants to a presence of oxidative stress.

Keywords: As stress; chlorophyll content; growth; leaf gas-exchange; lipid peroxidation; peroxidase; water relations
Subjects: arsenic, chlorophyll, water relations; bean, arsenic, chlorophyll; carotenoids, arsenic; chlorophylls, arsenic; cultivar and genotype differences, arsenic; gas exchange, arsenic; lipid peroxidation; malondialdehyde; nutrient medium Hoagland and Arnon; peroxidase; reactive oxygen species (ROS); relative water content; stomatal conductance, arsenic; superoxide dismutase; thiobarbituric acid; transpiration rate; water potential; water relations

Received: May 13, 2004; Accepted: August 27, 2004; Published: June 1, 2005  Show citation

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Stoeva, N., Berova, M., & Zlatev, Z. (2005). Effect of arsenic on some physiological parameters in bean plants. Biologia plantarum, 49(2), 293-296. doi: 10.1007/s10535-005-3296-z
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