biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 49:297-299, 2005 | DOI: 10.1007/s10535-005-7299-6

Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro

T. E. Sotiropoulos1,*, G. N. Mouhtaridou1, T. Thomidis1, V. Tsirakoglou2, K. N. Dimassi2, I. N. Therios2
1 N.AG.RE.F., Pomology Institute, Naoussa, Greece
2 Laboratory of Pomology, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki, Greece

The effects of five different N-sources (KNO3 + NH4NO3 = control, KNO3, NH4NO3, NH4H2PO4, L-alanine) on growth, nutritional status, chlorophyll (Chl) content, and photosynthetic parameters of the apple rootstock MM 106 shoots cultured in vitro were investigated. In comparison to all the other treatments, control explants grown on a MS medium containing KNO3 + NH4NO3 had the highest fresh mass, Chl content, net photosynthetic rate, transpiration rate, and stomatal conductance.

Keywords: cell proliferation; ion contents; micropropagation; net photosynthetic rate; nitrogen; stomatal conductance; transpiration rate
Subjects: amino acids; apple rootstock (EM 26, MM 106); cell proliferation; chlorophylls, nitrogen; gas exchange, nitrogen; in vitro culture, mass propagation; in vitro culture, nitrogen sources and ion contents; ion contents, in vitro culture; Malus domestica; nitrogen sources (KNO3, NH4NO3, NH4H2PO4, L-alanine), photosynthesis, ion contents in in vitro culture; nutrient medium MS (Murashige and Skoog); ribulose-1,5-bisphosphate carboxylase/oxygenase; rootstock, apple; stomatal conductance, nitrogen; transpiration rate

Received: December 10, 2003; Accepted: January 19, 2005; Published: June 1, 2005  Show citation

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Sotiropoulos, T.E., Mouhtaridou, G.N., Thomidis, T., Tsirakoglou, V., Dimassi, K.N., & Therios, I.N. (2005). Effects of different N-sources on growth, nutritional status, chlorophyll content, and photosynthetic parameters of shoots of the apple rootstock MM 106 cultured in vitro. Biologia plantarum, 49(2), 297-299. doi: 10.1007/s10535-005-7299-6
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