biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:309-312, 2005 | DOI: 10.1007/s10535-005-0312-2

Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: Host's RNA degradation

L. Šindelář1, M. Šindelářová1,*
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1, Prague 6, Czech Republic

Tobacco plants infected with the potato virus Y (PVY) were studied during the acute-infection period. The control enzymes of metabolic pathway of host's RNA degradation tending to biosynthesis of PVY-RNA, its coarse/fine regulation and content of host's RNA were monitored. Activities of ribonucleases, phosphomonoesterases and phosphodiesterases in both the crude homogenates and the partially purified enzyme preparations from the diseased leaves were markedly increased when compared to the tissues from healthy plants. The curves of enzyme activities positively correlated with the multiplication curve of the PVY and negatively correlated with the decreased contents of host's RNA. The enzyme activity in homogenate samples did not significantly differ from the corresponding purified enzyme preparations.

Keywords: Nicotiana tabacum L.; phosphodiesterase; phosphomonoesterase; potato virus Y; ribonuclease; RNA content
Subjects: Nicotiana tabacum; phosphodiesterase; phosphomonoesterase; potato virus Y; ribonuclease; tobacco, potato virus Y; virus

Received: June 23, 2004; Accepted: October 1, 2004; Published: June 1, 2005  Show citation

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Šindelář, L., & Šindelářová, M. (2005). Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: Host's RNA degradation. Biologia plantarum, 49(2), 309-312. doi: 10.1007/s10535-005-0312-2
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    References

    1. Cheo, P.C.: Effect in different plant species of continuous light and dark treatment on tobacco mosaic virus replicating capacity.-Virology 46: 256-265, 1971. Go to original source...
    2. Chersi, A., Bernardi, A., Bernardi, G.: Studies on acid hydrolases.-Biochim. biophys. Acta 129: 12-22, 1966. Go to original source...
    3. Clark, M.F., Adams, A.N.: Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.-J. gen. Virol. 34: 473-483, 1977. Go to original source...
    4. Diener, T.O.: Virus infection and other factors affecting ribonuclease activity of plant leaves.-Virology 14: 177-189, 1961. Go to original source...
    5. Farkas, G.L.: Ribonucleases and ribonucleic acid breakdown.-In: Parthier, B., Boulter, D. (ed.): Encyclopedia of Plant Physiology. Vol. 14B. Pp. 224-262. Springer-Verlag, Berlin-Heidelberg-New York 1982. Go to original source...
    6. Fraser, R.S.S.: Effects of two strains of tobacco mosaic virus on growth and RNA content of tobacco leaves.-Virology 47: 261-269, 1972. Go to original source...
    7. Green, P.J.: The ribonucleases of higher plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 421-445, 1994. Go to original source...
    8. Karlson, P.: Kurzes Lehrbuch der Biochemie für Mediziner und Naturwissenschaftler.-Georg Thieme Verlag, Stuttgart 1981.
    9. Leiser, R., Richter, J.: Reinigung und einige Eigenschaften des Kartoffel-Y-Virus.-Arch. Phytopathol. Pflanzenschutz 14: 337-350, 1978. Go to original source...
    10. Mančal, P.: Methods of Enzyme Immunoassay.-ÚSOL, Prague 1987.
    11. Nishimura, M., Graham, D., Akazawa, T.: Isolation of intact chloroplasts and other cell organelles from spinach leaf protoplasts.-Plant. Physiol. 58: 309-314, 1976. Go to original source...
    12. Oxelfelt, P.: Development of systemic tobacco mosaic virus infection. II. RNA metabolism in systemically infected leaves.-Phytopathol. Z. 71: 247-256, 1971. Go to original source...
    13. Randles, J.W.: Ribonuclease isozymes in Chinese cabbage systemically infected with turnip yellow mosaic virus.-Virology 36: 556-563, 1968. Go to original source...
    14. Reddi, K.K.: Studies on tobacco leaf ribonuclease. III. Its role in the synthesis of tobacco mosaic virus nucleic acid.-Biochim. biophys. Acta 33: 164-169, 1959. Go to original source...
    15. Reddi, K.K.: Studies on the formation of tobacco mosaic virus ribonucleic acid: II. Degradation of host ribonucleic acid following infection.-Proc. nat. Acad. Sci. USA 50: 75-81, 1963. Go to original source...
    16. Šindelář, L., Šindelářová, M., Čeřovská, N., HanuŠová, M.: Changes in ribonuclease and glucose-6-phosphate dehydrogenase activities during PVY-RNA biosynthesis in potato leaf discs.-Biol. Plant 32: 119-127, 1990. Go to original source...
    17. Šindelá řová, M., Šindelář, L.: Influence of antiviral factor on tobacco mosaic virus RNA biosynthesis in tobacco.-Biol. Plant. 46: 95-100, 2003.
    18. Š indelá řová, M., Šindelář, L.: Changes in glucose-6-phosphate dehydrogenase, ribonucleases, esterases and contents of viruses in potato virus Y infected tobacco superinfected with tobacco mosaic virus.-Biol. Plant. 47: 99-104, 2003/4.
    19. Šindelářová, M., Šindelář, L.: TMV-RNA biosynthesis in the light-green and dark-green regions of tobacco leaves.-Biol. Plant. 48: 419-423, 2004. Go to original source...
    20. Šindelářová, M., Šindelář, L., Burketová, L.: Dynamic changes in the activities of glucose-6-phosphate dehydrogenase, ribulosebisphosphate carboxylase and ribonuclease in tobacco leaves, leaf discs and mesophyll protoplasts in relatioon to TMV multiplication.-Physiol. mol. Plant Pathol. 51: 99-109, 1997. Go to original source...
    21. Šindelářová, M., Šindelář, L., Burketová, L.: Correlation between activity of ribonucleases and potato virus Y biosynthesis in tobacco plants.-Physiol. mol. Plant Pathol. 57: 191-199, 2000. Go to original source...
    22. Šindelářová, M., Šindelář, L., Burketová, L.: Glucose-6-phosphate dehydrogenase, ribonucleases and esterases upon tobacco mosaic virus infection and benzothiodiazole treatment in tobacco.-Biol. Plant. 45: 423-432, 2002. Go to original source...
    23. Suzuki, T., Taniguchi, Y.: Degradation of host high molecular weight RNA in barley leaves infected with barley stripe mosaic virus.-Phytopathol. Z. 77: 55-64, 1973. Go to original source...
    24. Wilson, C.M.: Plant nucleases.-Annu. Rev. Plant Physiol. 26: 187-208, 1975. Go to original source...
    25. Wyen, H.V., Udvardy, J., Erdei, S., Farkas, G.L.: The level of a relatively purine-specific ribonuclease increases in virus-infected hypersensitive or mechanically injured tobacco leaves.-Virology 48: 337-341, 1972. Go to original source...

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