biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:313-316, 2005 | DOI: 10.1007/s10535-005-3316-z

Use of a simple semiquantitative method for appraisal of green fluorescent protein gene expression in transgenic tobacco plants

M. Hraška2, S. Rakouský2,*, T. Kocábek1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
2 Faculty of Biological Sciences, University of South Bohemia, České Budějovice, Czech Republic

We have applied a simple method for evaluation of gfp gene expression in plants using a CCD camera and computerized processing of images. Transgenic tobacco plants were obtained by Agrobacterium tumefaciens-mediated transfer of plasmid T-DNA bearing a m-gfp5-ER sequence governed by the 35S promoter together with the nptII selectable marker gene. Presence of the gfp gene in plants was confirmed by a polymerase chain reaction method. Mean brightness values measured using image analysis software showed differences between transgenic and control plants and suggest the possibility of rapid selection of transgenic individuals among regenerants and their progenies.

Keywords: CCD camera; computerized processing; gfp expression; image analysis; Nicotiana tabacum
Subjects: Agrobacterium tumefaciens; gene transfer; green fluorescent protein, gfp gene expression; image analysis, gfp gene expression; Nicotiana tabacum; polymerase chain reaction (PCR); Southern blot analysis; transgenic plants

Received: July 14, 2004; Accepted: November 24, 2004; Published: June 1, 2005  Show citation

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Hraška, M., Rakouský, S., & Kocábek, T. (2005). Use of a simple semiquantitative method for appraisal of green fluorescent protein gene expression in transgenic tobacco plants. Biologia plantarum, 49(2), 313-316. doi: 10.1007/s10535-005-3316-z
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