biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:341-346, 2005 | DOI: 10.1007/s10535-005-0005-x

Propagation of Haemaria discolor via in vitro seed germination

Y.-J. Shiau1, S. M. Nalawade2, C.-N. Hsai1, H.-S. Tsay2,*
1 Department of Agronomy, Agricultural Research Institute, Wufong, Taichung, Taiwan
2 Institute of Biotechnology, Chaoyang University of Technology, Wufong, Taichung, Taiwan

In vitro propagation protocol for Haemaria discolor (Ker) Lindl. var. dawsoniana by artificial cross-pollination and asymbiotic germination of seeds has been developed. Fruit set (100 %) was obtained when the pollinia and ovules of various aged flowers were used for pollination. In vitro germination of seeds obtained from capsules of various ages was achieved on half-strength Murashige and Skoog's (MS) medium supplemented with 3 % sucrose and 0.85 % agar. The germinated seedlings were cultured on half-strength MS medium with 0.2 % activated charcoal, 8 % banana homogenate, 0.1 mg dm-3 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ) and 1 mg dm-3 α-naphthaleneacetic acid (NAA). Ninety-six percent of plantlets survived after hardening in greenhouse.

Keywords: in vitro propagation; Jewel orchid; pollination
Subjects: auxins; ex vitro transfer; flower; germination; Haemaria discolor; in vitro culture, acclimation, adaptation, to ex vitro transfer; in vitro culture, micropropagation; Juwel orchid, in vitro seed germination; nutrient medium MS (Murashige and Skoog); pollen germination; thidiazuron (TDZ)

Received: January 13, 2004; Accepted: March 3, 2005; Published: September 1, 2005  Show citation

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Shiau, Y.-J., Nalawade, S.M., Hsai, C.-N., & Tsay, H.-S. (2005). Propagation of Haemaria discolor via in vitro seed germination. Biologia plantarum, 49(3), 341-346. doi: 10.1007/s10535-005-0005-x
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