biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:371-380, 2005 | DOI: 10.1007/s10535-005-0010-0

Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants

L. Vrba1,*, J. Matousek1
1 Department of Molecular Genetics, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Ceske Budejovice, Czech Republic

A modified plant 7SL RNA gene from Arabidopsis thaliana designated AHIIA63M was introduced into potato plants via Agrobacterium-mediated transformation. No transgenic plants could be obtained using pGPTV-based binary vectors where AHIIA63M gene driven by polIII promoter was located close to the polII promoter of the selection gene. Special binary vectors with matrix attachment region (MAR) elements had to be used for transformation to insulate polII and polIII promoters within T-DNA. The level of AHIIA63M RNA in transgenic plants was lower than the levels of transcripts of transgenes driven by RNA polymerase II. The level of AHIIA63M transcript in transgenic potato plants was tissue specific. The highest expression was detected in roots and gynoecium and the lowest in tubers. Moreover, non-specific promoter activity within the MAR element was revealed. This activity contributed to AHIIA63M transcription. This is the first report of expression of a modified 7SL RNA gene in transgenic plants and promoter activity within the MAR element.

Keywords: RNA polymerase III; polIII expression cassette; MAR element
Subjects: Agrobacterium tumefaciens; Arabidopsis thaliana; beta-glucuronidase; matrix attachement region, MAR element; nuclear DNA content; potato, transgenic plants; reverse transcriptase polymerase chain reaction; RNA polymerase III; Solanum tuberosum; transgenic plants; viroids

Received: June 14, 2004; Accepted: January 4, 2005; Published: September 1, 2005  Show citation

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Vrba, L., & Matousek, J. (2005). Expression of modified 7SL RNA gene in transgenic Solanum tuberosum plants. Biologia plantarum, 49(3), 371-380. doi: 10.1007/s10535-005-0010-0
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