biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:395-404, 2005 | DOI: 10.1007/s10535-005-0014-9

The effects of ozone on growth and stomatal response in the F2 generation of hybrid poplar (Populus trichocarpa × Populus deltoides)

S. Y. Woo1,*, T. M. Hinckley2
1 Department of Environmental Horticulture, University of Seoul, Dongdaemun-Gu, Seoul, Republic of Korea
2 Tree Physiology Lab., College of Forest Resources, University of Washington, Seattle, USA

Thirty-six F2 hybrid poplar (Populus trichocarpa × P. deltoides) clones were fumigated with ozone to record its effects on growth, correlate them with stomatal response and screen for ozone sensitivity. Fumigation was applied for 6 to 9 h each day for approximately 3 months at ozone concentrations of 85 to 128 μg g-1 using open-top chambers. Height, diameter, number of leaves, stomatal conductance, transpiration rate, total biomass, biomass components and root/shoot ratios were reduced by ozone stress. Percent of leaf fall in ozone-treated plants was nearly three times higher than in control plants exposed to charcoal-filtered air. Leaf senescence, because of ozone exposure, did not appear to be associated with reduced biomass production. Some clones had a high percentage of leaf-fall with ozone exposure, but were able to maintain total biomass production near that of the control. Their response may be an example of an ability to adjust or compensate for ozone damage. There was no significant or consistent relationship between stomatal conductance and total biomass or the change in stomatal conductance as a result of ozone exposure and the change in total biomass. Taken together, these results suggest that effects of ozone on poplar growth cannot be solely correlated to changes in stomatal conductance, more physiological and biochemical parameters should be examined.

Keywords: leaf senescence; open top chamber; relative growth rate; stomatal conductance; transpiration rate
Subjects: gas exchange, stomatal conductance, ozone; growth analysis, plant development, biomass and yield enhancement; leaf plastochron index; leaf senescence; open top chamber (OTC); ozone, growth, stomata; poplar, F2 generation of hybrid; Populus trichocarpa × Populus deltoides; stomatal conductance, ozone; transpiration rate

Received: February 24, 2003; Accepted: November 21, 2003; Published: September 1, 2005  Show citation

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Woo, S.Y., & Hinckley, T.M. (2005). The effects of ozone on growth and stomatal response in the F2 generation of hybrid poplar (Populus trichocarpa × Populus deltoides). Biologia plantarum, 49(3), 395-404. doi: 10.1007/s10535-005-0014-9
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