biologia plantarum

Biologia plantarum 49:415-417, 2005 | DOI: 10.1007/s10535-005-0018-5

An efficient and rapid in vitro regeneration system for metal resistant cotton

E. Bicakci¹, A. R. Memon^2,*

¹ Department of Biology, Yildiz Technical University, Istanbul, Turkey

² Research Institute for Genetic Engineering and Biotechnology, TUBITAK, Gebze, Kocaeli, Turkey

In this report we describe the most suitable protocol for callus formation and plant regeneration for cotton. We screened 15 cotton (Gossypium hirsutum L.) genotypes for metal resistance and two of them, Nazilli M-503 (M503) Nazilli 143 (N-143) selected as Cd, Cu and Ni resistant. The cotyledonary nodes from these genotypes were the best explants for regeneration of shoots (more than 90 %) and roots (50 to 70 %). Shoot apex also gave good shoot regeneration (more than 90 %) but their root regeneration efficiency was low (35 %). These results show that Murashige and Skoog (MS) media containing 0.44 μM naphthaleneacetic acid (NAA) and 0.98 μM indole-3-butyric acid (IBA) was the most suitable recipe for getting high shoot and root regeneration from cotyledonary nodes of N-143 and M503 cotton genotypes.

Keywords: callus; Gossypium hirsutum; heavy metal tolerant plants; plant regeneration

Subjects: auxins; cotton, metal resistance; cultivar and genotype differences, metal resistance; cytokinins; gibberellic acid, gibberellins; Gossipiun hirsutum; heavy metals; in vitro culture, callus induction; in vitro culture, regeneration, organogenesis, morphogenesis; nutrient medium MS (Murashige and Skoog)

Received: August 15, 2003; Accepted: November 3, 2004; Published: September 1, 2005  Show citation