biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:471-474, 2005 | DOI: 10.1007/s10535-005-0032-7

Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants

M. ©indelářová1,*, L. ©indelář1, N. Wilhelmová1, D. Procházková1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

Effects of the infection with tobacco mosaic virus (TMV) and potato virus Y (PVY) on chloroplasts from susceptible tobacco plants were determined. Changes in ribonucleases (RNases), phosphomonoesterase (PME), phosphodiesterase (PDE), glucose-6-phosphate dehydrogenase (G6P DH), 6-phosphogluconate dehydrogenase (6PG DH), glucokinase (GK), and fructokinase (FK) activities in thylakoid/envelope and stroma fractions were studied. Slight increase in the activities of PME, PDE, G6P DH and 6PG DH of thylakoid/envelope fraction as well as of RNases, PME, PDE, G6P DH, 6PG DH, GK and FK of stroma fraction was found in chloroplasts isolated from leaf tissues infected with PVY. Infection with TMV produced higher increase in enzymes activities in chloroplasts; especially, PME, G6P DH and 6PG DH in fraction of thylakoid/envelope, and RNases, PME, PDE, G6P DH, 6PG DH, and GK in stroma fraction.

Keywords: chlorophylls; Nicotiana tabacum L. cv. Samsun; protein; RNA
Subjects: caffeic acid; catechine; chloroplast, PVY and TMV infected tobacco; coniferyl alcohol; guaiacol peroxidase; isoferulic acid; lignin; omorika, guaiacol peroxidase, pollution stress; peroxidase; Picea omorika; pollution stress; polyvinylpyrrolidone

Received: November 16, 2004; Accepted: April 25, 2005; Published: September 1, 2005  Show citation

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©indelářová, M., ©indelář, L., Wilhelmová, N., & Procházková, D. (2005). Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants. Biologia plantarum, 49(3), 471-474. doi: 10.1007/s10535-005-0032-7
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