biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:605-607, 2005 | DOI: 10.1007/s10535-005-0056-z

Random amplified DNA polymorphism of Nicotiana tabacum L. cultivars

H. Y. Zhang1,*, X. Z. Liu1, C. S. He2, C. M. Zheng3
1 Southwest Forestry College, Kunming, Yunnan Province, P.R. China
2 The South Center of Tobacco Breeding Research of China, Yuxi, Yunnan, P.R. China
3 College of Agronomy, South China University of Tropic Agriculture, Dangzhao, Hainan, P.R. China

The polymorphism, similarities and relationships among Nicotiana tabacum L. cultivars were assessed with RAPD analyses. One hundred and forty-nine bands were detected, of which 94 were polymorphic (63.1 %). A primer distinguishing all of the tested cultivars was found. High similarity between cultivars was revealed, and cultivar relationships were estimated through cluster analysis (UPGMA) based on RAPD data.

Keywords: flue-cured tobacco; genetic diversity; RAPD; UPGMA
Subjects: cultivar and genotype differences, DNA polymorphism; dendrogram, cladogram, phenogram, phylogenetic tree; flue-cured tobacco; genetic diversity; Nicotiana tabacum; randomly amplified polymorphic DNA (RAPD); tobacco, RAPD

Received: August 15, 2003; Accepted: October 11, 2004; Published: December 1, 2005  Show citation

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Zhang, H.Y., Liu, X.Z., He, C.S., & Zheng, C.M. (2005). Random amplified DNA polymorphism of Nicotiana tabacum L. cultivars. Biologia plantarum, 49(4), 605-607. doi: 10.1007/s10535-005-0056-z
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