biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:617-619, 2005 | DOI: 10.1007/s10535-005-0059-9

Influence of lead on membrane permeability and lipoxygenase activity in lupine roots

R. Rucinska1, E. A. Gwozdz1,*
1 Laboratory of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Poznan, Poland

Lead nitrate at concentration of 150 mg dm-3 inhibits root growth of Lupinus luteus seedlings by bout 20 %, which is accompanied by an increase of K+ leakage from the root cells. Non-denaturing isoelectric focusing in polyacrylamide slab gel has shown that lead stimulates the activity of most lipoxygenase isoenzymes and induces one additional isoenzyme with pI 6.9.

Keywords: K+ leakage; lipid peroxidation; Lupinus luteus
Subjects: lead, membrane permeability; lipid peroxidation; lipoxygenase; lupine, roots; Lupinus luteus; malondialdehyde; membrane permeability, lead; potassium, K+ leakage; root, membrane permeability

Received: November 10, 2003; Accepted: April 15, 2005; Published: December 1, 2005  Show citation

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Rucinska, R., & Gwozdz, E.A. (2005). Influence of lead on membrane permeability and lipoxygenase activity in lupine roots. Biologia plantarum, 49(4), 617-619. doi: 10.1007/s10535-005-0059-9
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    References

    1. Bhattacharejee, S.: Membrane lipid peroxidation, free radical scavengers and ethylene evolution in Amaranthus as affected by lead and cadmium.-Biol. Plant. 40: 131-135, 1998. Go to original source...
    2. Bradford, M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. De Vos, C.H.R., Schat, H., Vooijs, R., Ernst, W.H.O.: Copper-induced damage to the permeability barrier in roots of Silene cucubalus.-J. Plant Physiol. 135: 164-169, 1989. Go to original source...
    4. Gaillard, T., Chan, H.W.S.: Lipoxygenases.-In: Stumpf, P.K. (ed.): The Biochemistry of Plants: a Comprehensive Treatise. Vol. 4 Lipids: Structure and Function. Pp. 131-161. Academic Press, New York 1980. Go to original source...
    5. Gonnelli, C., Galardi, F., Gabbrielli R.: Nickel and copper tolerance and toxicity in three Tuscan populations of Silene paradoxa.-Physiol. Plant. 113: 507-514, 2001. Go to original source...
    6. Halliwell, B., Gutteridge, J.M.C.: Free Radicals in Biology and Medicine.-Clarendon Press, Oxford 1989.
    7. Hartley-Whitaker, J., Ainsworth, G., Meharg, A.A.: Copper-and arsenate-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity.-Plant Cell Environ. 24: 713-722, 2001. Go to original source...
    8. Heinisch, O., Kowalski, E., Ludwig, H., Tauscher, B.: Staining for soybean lipoxygenase activity in electrophoretic gels.-Fett/Lipid 5: 193-184, 1996. Go to original source...
    9. Hendry, G.A.F., Grime, J.P.: Methods in Comparative Plant Ecology.-Chapman & Hall, London 1993. Go to original source...
    10. Jackowski, G., Przymusinski, R.: The resolution and biochemical characterization of subcomplexes of the main light-harvesting chlorophyll a/b-protein complex of photosystem II (LHC II).-Photosynth. Res. 43: 41-48, 1995. Go to original source...
    11. Maccarrone, M., Van Aarie, P.G.M., Veldink, G.A., Vliegenthart J.F.G.: In vitro oxygenation of soybean biomembranes by lipoxygenase-2.-Biochim. biophys. Acta 1190: 164-169, 1994. Go to original source...
    12. Mishra, A., Choudhuri, M.A.: Effects of salicylic acid on heavy metal-induced membrane deterioration mediated by lipoxygenase in rice.-Biol. Plant. 42: 409-415, 1999. Go to original source...
    13. Shah, K., Kumar, R.G., Verma, S., Dubey, R.S.: Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings.-Plant Sci. 161: 1135-1144, 2001. Go to original source...
    14. Shainberg, O., Rubin, B., Rabinowitch, H.D., Libal, Y., Tel-Or E.: Acclimation of beans to oxidative stress by treatment with sublethal iron levels.-J. Plant Physiol. 157: 93-99, 2000. Go to original source...
    15. Siedow, J.N.: Plant lipoksygenase: structure and function.-Annu. Rev. Plant Physiol. Plant mol.Biol. 42: 145-188, 1991. Go to original source...
    16. Weckx, J.E.J., Clijsters, H.M.M.: Oxidative damage and defense mechanisms in primary leaves of Phasoeolus vulgaris as a result of root assimilation of toxic amounts of cooper.-Physiol. Plant. 96: 506-512, 1996. Go to original source...
    17. Weckx, J.E.J., Clijsters, H.M.M.: Zn phytotoxicity induces oxidative stress in bean leaves Phaseolus vulgaris.-Plant Physiol. Biochem. 35: 405-410, 1997.

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