biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:625-628, 2005 | DOI: 10.1007/s10535-005-0062-1

Effect of γ-radiation on development, yield and quality of microtubers in vitro in Solanum tuberosum L.

H. Z. Li1,2, W. J. Zhou1,3,*, Z. J. Zhang1,2, H. H. Gu1, Y. Takeuchi3, K. Yoneyama3
1 Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
2 Department of Environment and Safety Engineering, North University of China, Taiyuan, China
3 Center for Research on Wild Plants, Utsunomiya University, Utsunomiya, Japan

Explants obtained from in vitro-propagated plantlets of two potato cultivars, Shepody and Atlantic, were treated with five doses of γ-radiation (0, 2, 4, 6 and 8 Gy) to investigate the stimulating effects of low irradiation on the production and quality of microtubers in vitro. Microtubers of both cultivars treated with γ-radiation initiated 5 d earlier than in the non-irradiated control. The whole period of microtuberization was prolonged by 10 - 15 d with 4, 6 and 8 Gy irradiation treatment for cv. Atlantic. Irradiation of the plantlets (4 Gy) led to a significant increase not only in the microtuber number (116.7 and 34.5 % over the control) but also in the fresh mass (77.6 and 23.2 % in Shepody and Atlantic, respectively). Low dose irradiation (2 - 4 Gy) increased the starch content of microtubers. High doses (6 - 8 Gy) enhanced ascorbic acid and reducing sugar contents. 4 - 6 Gy doses also effectively increased the protein contents of microtubers.

Keywords: ascorbic acid; microtuberization; plantlet; potato; protein; reducing sugar; starch
Subjects: ascorbate peroxidase; cultivar and genotype differences, potato microtubers; gamma-radiation, yield, potato microtubers; growth analysis, plant development, biomass and yield enhancement; in vitro culture, microtubers; microtuberization; potato microtubers; protein, γ-irradiation, potato microtubers; Solanum tuberosum; sugars, reducing, γ-irradiation, potato microtubers

Received: March 2, 2004; Accepted: October 25, 2004; Published: December 1, 2005  Show citation

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Li, H.Z., Zhou, W.J., Zhang, Z.J., Gu, H.H., Takeuchi, Y., & Yoneyama, K. (2005). Effect of γ-radiation on development, yield and quality of microtubers in vitro in Solanum tuberosum L. Biologia plantarum, 49(4), 625-628. doi: 10.1007/s10535-005-0062-1
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