biologia plantarum

Biologia plantarum 50:107-110, 2006 | DOI: 10.1007/s10535-005-0081-y

Efficient and repetitive production of leaf-derived somatic embryos of Oncidium

Y. J. Su¹, J. T. Chen^2,3,*, W. C. Chang¹

¹ Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

² Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China

³ Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China

Oncidium cultivars gave different embryogenic responses of leaf explants when affected by auxins (2,4-D, IAA, IBA and NAA), cytokinins (2iP, BA, kinetin, TDZ and zeatin), sucrose, NaH₂PO₄, casein hydrolysate, peptone, and glutamine. The best embryogenic responses of cv. Sweet Sugar were at 20 g dm^-3 sucrose, 85 mg dm^-3 NaH₂PO₄ and 3 mg dm^-3 kinetin, respectively. The development of somatic embryos on leaf explants of cv. Sweet Sugar was delayed for about 10 - 20 d in comparison with cv. Gower Ramsey. On growth regulator-free medium, about 40 % of leaf derived embryos of cv. Gower Ramsey were fused together in their basal parts and so called multiple-state embryos. However, under the same condition, the embryos of cv. Sweet Sugar were all in multiple-state form.

Keywords: direct somatic embryogenesis; genotype; medium composition

Subjects: auxins; cultivar and genotype differences; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Murashige and Skoog; Oncidium sp.; thidiazuron (TDZ)

Received: September 4, 2003; Accepted: August 20, 2005; Published: March 1, 2006  Show citation