biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:111-113, 2006 | DOI: 10.1007/s10535-005-0082-x

Assessment of genetic diversity in Coscinium fenestratum

S. Narasimhan1, P. Padmesh2, G. M. Nair1,*
1 Department of Botany, University of Kerala, Kariavattom, Thiruvananthapuram, Kerala, India
2 Tropical Botanic Garden & Research Institute, Palode, Thiruvananthapuram, Kerala, India

Random amplified polymorphic DNA (RAPD) markers were used to assess the genetic diversity of 14 individuals belonging to 7 populations of Coscinium fenestratum (Gaertn.) Colebr. (Menispermaceae). 18 decamer primers used for the analysis generated 99 scorable bands of which 79 were found to be polymorphic. Coefficient of similarity ranged from 0.6604 to 0.9809. Variation within population was slightly higher than between populations. Similarity between individuals within and between populations was found. Dendrogram was obtained by using unwieghed pair-group method analysis (UPGMA). Distinct accession also exhibited higher percentage of medicinally active compound.

Keywords: berberine; critically endangered species; ex situ conservation; RAPD
Subjects: berberine; Coscinium fenestratum; dendrogram, phylogenetic tree, phenogram; genetic diversity, variability; medicinal plants; random amplified polymorphic DNA (RAPD)

Received: September 29, 2003; Accepted: August 30, 2005; Published: March 1, 2006  Show citation

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Narasimhan, S., Padmesh, P., & Nair, G.M. (2006). Assessment of genetic diversity in Coscinium fenestratum. Biologia plantarum, 50(1), 111-113. doi: 10.1007/s10535-005-0082-x
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