biologia plantarum

Biologia plantarum 50:114-117, 2006 | DOI: 10.1007/s10535-005-0083-9

Effects of medium composition and culture duration on in vitro morphogenesis of sweet potato

L. Chen^1,*, A. Bhagsari¹, J. Carter¹

¹ Agricultural Research Station, Fort Valley State University, Fort Valley, USA

In vitro morphogenesis of sweet potato (Ipomoea batatas) shoot explants after cultures in callus initiation medium (CIM) with two sucrose contents and plant regeneration medium (PRM) with three growth regulator combinations for different durations was studied. After 4 weeks, explants on 5 % sucrose CIM had significantly more shoots but similar or lower root fresh mass and callus fresh mass than those on 3 % sucrose CIM subsequent to transfer for 6 weeks on all three PRM. Cultures transferred to growth regulator-free PRM after 4 and 12 weeks on 5 % sucrose CIM formed plants through organogenesis and embryogenesis, respectively. Embryogenic cultures from 4 weeks on CIM + 10 weeks on callus proliferation medium when transferred to PRM without growth regulator for 4 and 8 weeks produced multiple embryos in the prior and both embryos and shoot buds in the later.

Keywords: callus growth; Ipomoea batatas L. Lam; root growth; shoot regeneration

Subjects: auxins; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, organogenesis; in vitro culture, regeneration, proliferation, differentiation; Ipomoea batatas; nutrient medium, callus initiation medium; nutrient medium, Murashige and Skoog; root, rooting; sweet potato; thidiazuron (TDZ)

Received: October 27, 2003; Accepted: November 3, 2004; Published: March 1, 2006  Show citation