biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:123-126, 2006 | DOI: 10.1007/s10535-005-0085-7

In vitro organogenesis and plant formation in cucumber

N. Selvaraj2, A. Vasudevan1, M. Manickavasagam1, A. Ganapathi1,*
1 Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
2 Department of Botany, Periyar E.V.R. College (Autonomous), Tiruchirappalli, Tamil Nadu, India

In vitro organogenesis was achieved from callus derived from hypocotyl explants of Cucumis sativus L. cv. Poinsett 76. Calli were induced from hypocotyl explants excised from 7-d-old seedlings grown on Murashige and Skoog (MS) medium containing 87.64 µM sucrose, 0.8 % agar, 3.62 µM 2,4-dichlorophenoxy acetic acid and 2.22 µM 6-benzyladenine (BA). Regeneration of adventitious buds from callus (25 shoots explant-1) was achieved on MS medium supplemented with 8.88 µM BA, 2.5 µM zeatin and 10 % coconut water after two subcultures in the same medium at 30-d interval. Gibberellic acid (1.75 µM) favoured shoot elongation and indole 3-butyric acid (7.36 µM) induced rooting. Rooted plants were hardened and successfully established in soil.

Keywords: adventitious bud; callus; Cucumis sativus L.; hypocotyl
Subjects: auxins; cucumber; Cucumis sativus; cytokinins; gibberellic acid, gibberellins; hypocotyl; in vitro culture, callus growth and duration; in vitro culture, organogenesis; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog

Received: December 10, 2003; Accepted: October 9, 2004; Published: March 1, 2006  Show citation

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Selvaraj, N., Vasudevan, A., Manickavasagam, M., & Ganapathi, A. (2006). In vitro organogenesis and plant formation in cucumber. Biologia plantarum, 50(1), 123-126. doi: 10.1007/s10535-005-0085-7
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    References

    1. Chee, P.P.: High frequency somatic embryogenesis and recovery of fertile cucumber plants.-Sci. Hort. 25: 792-793, 1990. Go to original source...
    2. Chee, P.P., Tricoli, D.M.: Somatic embryogenesis and plant regeneration from cell suspension cultures of Cucumis sativus L.-Plant Cell Rep. 7: 274-277, 1988. Go to original source...
    3. Gomez, K.A., Gomez, K.A.: Statistical Procedures for Agricultural Research with Emphasis of Rice.-International Rice Research Institute, Los Banos 1976.
    4. Handley, L.W., Chambliss, O.L.: In vitro propagation of Cucumis sativus L.-Sci. Hort. 14: 22-23, 1979. Go to original source...
    5. Jia, S.R., Fu, Y.Y., Lin, Y.: Embryogenesis and plant regeneration from cotyledon protoplast culture of cucumber (Cucumis sativus L.).-Plant Physiol. 124: 393-398, 1986. Go to original source...
    6. Kim, S.G., Chang, J.R., Cha, H.C., Lee, K.W.: Callus growth and plant regeneration in diverse cultivars of cucumber (Cucumis sativus L.).-Plant Cell Tissue Org Cult. 12: 67-74, 1988. Go to original source...
    7. Lou, H., Kako, S.: Somatic embryogenesis and plant regeneration in cucumber.-Sci. Hort. 29: 906-909, 1994. Go to original source...
    8. Malepszy, S.: Cucumber (Cucumis sativus L.).-In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 6. Pp. 277-293. Springer-Verlag, Berlin 1988. Go to original source...
    9. Malepszy, S., Nadolska-Oreszyk, A.: In vitro culture of Cucumis sativus L. Regeneration of plantlets from callus formed by leaf explants.-Z. Pflanzenphysiol. 111: 273-276, 1983. Go to original source...
    10. Murashige, T., Skoog, F.: A revised medium for rapid growth and bio assays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    11. Nadolska-Oreszyk, A., Malepszy, S.: In vitro culture of Cucumis sativus L. VI. Histological analysis of leaf explants cultured on media with 2,4-D or 2,4,5-T.-Acta Soc. Bot. Pol. 56: 55-60, 1986. Go to original source...
    12. Nishibayashi, S., Kaneko, H., Hayakawa, T.: Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants.-Plant Cell Rep. 15: 809-814, 1996. Go to original source...
    13. Oreszyk, W., Malepszy, S.: In vitro culture of Cucumis sativus L. V. Stabilizing effects of glycine on leaf protoplasts.-Plant Cell Rep. 4: 269-273, 1985. Go to original source...
    14. Punja, Z.K., Abbas, N., Sarmento, G.G., Tang, F.A.: Regeneration of Cucumis sativus, vars, sativus and hardwickii, C. melo and C. metuliferous from explants through somatic embryogenesis and organogenesis. Influence of explant source, growth regulator regime and genotype.-Plant Cell Tissue Organ Cult. 21: 93-102, 1990. Go to original source...
    15. Raharjo, S.H.T., Hernandez, M.O., Zhang, Y.Y., Punja, Z.K.: Transformation of pickling cucumber with chitinase-encoding genes using Agrobacterium tumefaciens.-Plant Cell Rep. 15: 591-596, 1996. Go to original source...
    16. Selvaraj, N., Vengadesan, G., Vasudevan, A., Prem Anand, R., Ramesh Anbazhgan, V., Ganapathi, A.: Micropropagation of Cucumis sativus L. from field grown plants.-In: Maynard, D.N. (ed.): Proceedings of Cucurbitaceae 2002 Conference. Pp. 149-156. ISHS Publications, Naples 2002.
    17. Seo, S.H., Bai, D.G., Park, H.Y.: High frequency shoot regeneration from leaf explants of cucumber.-J Plant Biotechnol. 21: 51-54, 2000.
    18. Trulson, A.J., Shahin, E.H.: In vitro plant regeneration in the genus Cucumis.-Plant Sci. 47: 35-43, 1986. Go to original source...
    19. Wehner, T.C., Locy, R.D.: In vitro adventitious shoot and root formation of cultivars and lines of Cucumis sativus L.-Hort. Sci. 16: 759-760, 1981. Go to original source...
    20. Ziv, M., Gadasi, G.: Enhanced embryogenesis and plant regeneration from cucumber (Cucumis sativus L.) callus by activated charcoal in solid/liquid double layer cultures.-Plant Sci. 47: 115-112, 1986. Go to original source...

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