Biologia plantarum 50:127-130, 2006 | DOI: 10.1007/s10535-005-0086-6
Evaluation of genetic relationship in Typhonium species through random amplified polymorphic DNA markers
- 1 Plant Biotechnology Division, Regional Plant Resource Centre, Bhubaneswar, Orissa, India
Studies were undertaken to identify genetic relationships in three species of Typhonium and to evaluate the genetic variance within populations of Typhonium trilobatum, Typhonium roxburghii and Typhonium flagelliforme by using random amplified polymorphic DNA (RAPD) markers. A total of 193 distinct DNA fragments ranging from 0.2 to 3.2 kb, were amplified using 22 selected random decamer primers. The cluster analysis indicated that the three species of Typhonium formed two clusters: the first one consisted of T. trilobatum and T. roxburghii, the second one was represented by T. flagelliforme. A maximum similarity of 63 % was observed in T. trilobatum and T. roxburghii. T. flagelliforme shared up to 43 % similarity with T. trilobatum and T. roxburghii. The closest genetic distance was obtained within populations of different Typhonium species.
Keywords: Araceae; medicinal plant; RAPD
Subjects: dendrogram, phylogenetic tree, phenogram; medicinal plants; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD); Typhonium flagelliforme; Typhonium roxburghii; Typhonium trilobatum
Received: February 16, 2004; Accepted: April 29, 2005; Published: March 1, 2006 Show citation
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