biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:142-145, 2006 | DOI: 10.1007/s10535-005-0090-x

Secretion of a chitinase-like protein in embryogenic suspension cultures of Dactylis glomerata L.

M. I. Tchorbadjieva1,*, I. Y. Pantchev1
1 Department of Biochemistry, Faculty of Biology, Sofia University, Sofia, Bulgaria

A chitinase-like 32 kDa acidic protein with a potential chitinase activity has been identified in the medium of embryogenic suspension cultures of Dactylis glomerata L. using an antiserum raised against endochitinase EP3 from Daucus carota L. The presence of this protein discriminates between Dactylis glomerata L. embryogenic and nonembryogenic suspension cultures and thus could be possibly used as a marker for embryogenic potential.

Keywords: extracellular proteins; orchardgrass; somatic embryogenesis
Subjects: chitinase-like protein; Dactylis glomerata; 3,6-dichloro-o-anisic acid; in vitro culture, cell suspension cultures; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Schenk and Hildebrandt (SH); orchard grass; proteins

Received: April 23, 2004; Accepted: September 7, 2004; Published: March 1, 2006  Show citation

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Tchorbadjieva, M.I., & Pantchev, I.Y. (2006). Secretion of a chitinase-like protein in embryogenic suspension cultures of Dactylis glomerata L. Biologia plantarum, 50(1), 142-145. doi: 10.1007/s10535-005-0090-x
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