biologia plantarum

Biologia plantarum 50:152-155, 2006 | DOI: 10.1007/s10535-005-0093-7

Somatic embryogenesis and plant regeneration in diploid and triploid Arachis pintoi

H. Y. Rey^1,*, L. A. Mroginski¹

¹ Facultad de Ciencias Agrarias (UNNE), Instituto de Botanica del Nordeste (IBONE), Corrientes, Argentina

Plants of two cytotypes (2n=2x=20, and 2n=3x=30) of pinto peanut (Arachis pintoi Krapov. & W.C. Gregory) were regenerated through somatic embryogenesis. Embryogenic calli were induced from shoot tips or immature leaves dissected from in vitro growing plants. In the case of the diploid peanut the best somatic embryogenesis was achieved when shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with 10 mg dm^-3 Picloram (PIC) and 0.1 mg dm^-3 6-benzylaminopurine (BAP) or when explants from immature leaves were cultured on MS + 10 mg dm^-3 PIC + 0.01 mg dm^-3 BAP. In the case of triploid peanut the highest number of somatic embryos was obtained when shoot tips were cultured on MS + 10 mg dm^-3 PIC + 0.01 mg dm^-3 BAP or when immature leaves were cultured on MS + 20 mg dm^-3 PIC + 0.01 mg dm^-3 BAP. Somatic embryos were converted into plants by culture on MS + 0.01 mg dm^-3 naphthaleneacetic acid + 0.01 mg dm^-3 BAP. Plants were successfully transferred to pots in greenhouse.

Keywords: forage legume; growth regulators; in vitro cultivation; leaf culture; Leguminosae; shoot tip

Subjects: Arachis pintoi; auxins; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; ploidy

Received: May 17, 2004; Accepted: February 25, 2005; Published: March 1, 2006  Show citation