biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:169-173, 2006 | DOI: 10.1007/s10535-006-0002-8

Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis

J. T. Chen1,2,*, W. C. Chang3
1 Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China
2 Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China
3 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China

Leaf explants of Phalaenopsis amabilis var. formosa formed clusters of somatic embryos directly from epidermal cells without an intervening callus within 20 - 30 d when cultured on 1/2-strength modified Murashige and Skoog medium supplemented with 0.1, 1 and 3 mg dm-3 TDZ. Repetitive production of embryos involved secondary embryogenesis could be obtained by culturing segments of embryogenic masses on TDZ-containing media. Plantlet conversion from embryos was successfully achieved on regulator-free growth medium.

Keywords: moth orchid; naphthaleneacetic acid; thidiazuron
Subjects: auxins; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; moth orchid; nutrient medium, Murashige and Skoog; Phalaenopsis amabilis

Received: January 14, 2004; Accepted: March 20, 2005; Published: June 1, 2006  Show citation

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Chen, J.T., & Chang, W.C. (2006). Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biologia plantarum, 50(2), 169-173. doi: 10.1007/s10535-006-0002-8
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    References

    1. Arditti, J., Ernst, R. (ed.): Micropropagation of Orchids.-John Wiley & Son, New York 1993.
    2. Chen, J.T., Chang, C., Chang, W.C.: Direct somatic embryogenesis from leaf explants of Oncidium 'Gower Ramsey' and subsequent plant regeneration-Plant Cell Rep. 19: 143-149, 1999. Go to original source...
    3. Chen, J.T., Chang, W.C.: Effects of auxins and cytokinins on direct somatic embryogenesis from leaf explants of Oncidium 'Gower Ramsey'.-Plant Growth Regul. 34: 229-232, 2001. Go to original source...
    4. Chen, Y., Piluek, C.: Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis.-Plant Growth Regul. 16: 99-101, 1995. Go to original source...
    5. Chen, Y.C., Chang, C., Chang, W.C.: A reliable protocol for plant regeneration from callus culture of Phalaenopsis.-In vitro cell. dev. Biol. Plant 36: 420-423, 2000. Go to original source...
    6. Duan, J.X., Chen, H., Yazawa, S.: In vitro propagation of Phalaenopsis via culture of cytokinins-induced nodes.-J. Plant Growth Regul. 15: 133-137, 1996. Go to original source...
    7. Duncan, D.B.: Multiple range and multiple F test.-Biometrics 11: 1-42, 1955. Go to original source...
    8. Ernst, R.: Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae).-Plant Cell Tissue Organ Cult. 39: 273-275, 1994. Go to original source...
    9. Ishii, Y., Takamura, T., Goi, M., Tanaka, M.: Callus induction and somatic embryogenesis of Phalaenopsis.-Plant Cell Rep. 17: 446-450, 1998. Go to original source...
    10. Islam, M.O., Ichihashi, S.: Effects of sucrose, maltose and sorbitol on callus growth of Phalaenopsis, Doritaenopsis and Neofinetia.-J. jap. Soc. hort. Sci. 68: 1124-1131, 1999. Go to original source...
    11. Jensen, W.A.: Botanical Histochemistry.-Freeman, San Francisco 1962.
    12. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 495-497, 1962. Go to original source...
    13. Park, S.Y., Murthy, H.N., Paek, K.Y.: Rapid propagation of Phalaenopsis from floral stalk-derived leaves.-In Vitro cell. dev. Biol. Plant 38: 168-172, 2002. Go to original source...
    14. Su, Y.J., Chen, J.T., Chang, W.C.: Efficient and repetitive production of leaf-derived somatic embryos of Oncidium.-Biol. Plant. 50: in press, 2006. Go to original source...
    15. Tanaka, M., Hasegawa, A., Goi, M.: Studies on the clonal propagation of monopodial orchids by tissue culture. I. Formation of protocorm-like bodies from leaf tissues in Phalaenopsis and Vanda.-J. jap. Soc. hort. Sci. 44: 47-58, 1975. Go to original source...
    16. Teob, E.S. (ed.): Orchids of Asia.-Times Books International, Singapore 1989.
    17. Tokuhara, K., Masahiro, M.: Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds.-Plant Cell Rep. 13: 7-11, 1993. Go to original source...
    18. Tokuhara, K., Mii, M. Induction of embryogenic callus and cell suspension culture from shoot tips excised from flower stalk buds of Phalaenopsis (Orchidaceae).-In Vitro cell. dev. Biol. Plant 37: 457-461, 2001. Go to original source...
    19. Wang, H.C., Chen, J.T., Chang, W.C.: Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu.-Biol. Plant. 50: in press, 2006. Go to original source...
    20. Young, P.S., Murthy, H.N., Yoeup, P.K.: Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis.-Plant Cell Tissue Organ Cult. 63: 67-72, 2000. Go to original source...

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