biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:174-179, 2006 | DOI: 10.1007/s10535-006-0003-7

Thidiazuron stimulates adventitious shoot regeneration in different safflower explants

K. Radhika1, M. Sujatha2,*, T. Nageshwar Rao1
1 Acharya N.G. Ranga Agricultural University, Rajendranagar, Hyderabad, India
2 Directorate of Oilseeds Research, Rajendranagar, Hyderabad, India

Adventitious shoot regeneration from root, hypocotyl, cotyledon and primary leaf explants of safflower (Carthamus tinctorius L.) was studied. Shoot regeneration was promoted by benzyladenine (BA) + naphthaleneacetic acid (NAA), BA + indole-3-butyric acid (IBA), kinetin + NAA and thidiazuron (TDZ) + NAA incorporated in Murashige and Skoog (MS) basal medium. High frequency of shoot regeneration and high number of shoots per regenerating explant were obtained on a wide range of TDZ + NAA combinations. Proliferated shoots were elongated in MS + 0.5 mg dm-3 kinetin and well-developed shoots were rooted in half strength MS + 0.5 mg dm-3 NAA. Rooted shoots were successfully acclimatized and established in soil.

Keywords: auxins; Carthamus tinctorius L.; cytokinins; in vitro culture
Subjects: auxins; Carthamus tinctorius; cytokinins; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; safflower

Received: May 28, 2004; Accepted: March 4, 2005; Published: June 1, 2006  Show citation

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Radhika, K., Sujatha, M., & Nageshwar Rao, T. (2006). Thidiazuron stimulates adventitious shoot regeneration in different safflower explants. Biologia plantarum, 50(2), 174-179. doi: 10.1007/s10535-006-0003-7
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    References

    1. Bhattacharya, J., Renukdas, N.N., Khuspe, S.S., Rawal, S.K.: Multiple shoot regeneration from immature embryo explants of papaya.-Biol. Plant. 47: 327-331, 2003/4. Go to original source...
    2. George, E.F.: Plant Propagation by Tissue Culture. Part I. The Technology.-Exegetics Ltd, Edington 1993.
    3. George, L., Rao, P.S.: In vitro multiplication of safflower (Carthamus tinctorius L.) through tissue culture.-Proc. indian nat. Sci. Acad. B 48: 791-794, 1982.
    4. Hosseini-Nasr, M., Rashid, A.: Thidiazuron-induced high-frequency shoot regeneration from root region of Robinia pseudoacacia L. seedlings.-Biol. Plant. 47: 593-596, 2003/4. Go to original source...
    5. Huetteman, C.A., Preece, J.E.: Thidiazuron: a potent cytokinin for woody plant tissue culture.-Plant Cell Tissue Organ Cult. 33: 105-119, 1993. Go to original source...
    6. Mandal, A.K.A., Chatterji, A.K., Gupta, S.D.: Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower.-Plant Cell Tissue Organ Cult. 43: 287-289, 1995. Go to original source...
    7. Mandal, A.K.A., Gupta, S.D.: Somatic embryogenesis of safflower: influence of auxin and ontogeny of somatic embryos.-Plant Cell Tissue Organ Cult. 72: 27-31, 2003. Go to original source...
    8. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-497, 1962. Go to original source...
    9. Nikam, T.D., Shitole, M.G.: In vitro culture of safflower L. cv. Bhima: initiation, growth optimization and organogenesis.-Plant Cell Tissue Organ Cult. 55: 15-22, 1999. Go to original source...
    10. Orlikowska, T.K., Dyer, W.E.: In vitro regeneration and multiplication of safflower (Carthamus tinctorius L.).-Plant Sci. 93: 151-157, 1993. Go to original source...
    11. Prasad, B.R., Khadeer, M.A., Seeta, P., Anwar, S.Y.: In vitro induction of androgenic haploids in safflower (Carthamus tinctorius L.).-Plant Cell Rep. 10: 48-51, 1991. Go to original source...
    12. Sujatha, M., Suganya, A.: In vitro organogenic comparison of different seedling tissues of safflower (Carthamus tinctorius L.).-Sesame Safflower Newslett. 11: 85-90, 1996.
    13. Ying, M., Dyer, W.E., Bergman, J.W.: Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv. Centennial.-Plant Cell Rep. 11: 581-585, 1992. Go to original source...

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