biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:187-192, 2006 | DOI: 10.1007/s10535-006-0005-5

Characterization of the Ferredoxin-Gogat gene (OsGog2 clone) expression in rice

M. Mattana1,*, E. Biazzi1, A. Bertani1, I. Coraggio1
1 Istituto di Biologia e Biotecnologia Agraria, CNR, Milano, Italy

Ferredoxin-dependent glutamate synthase (Fd-Gogat; EC 1.4.7.1) in leaf and root plastids is the last enzyme involved in the pathway of nitrate assimilation in higher plants. Arabidopsis thaliana expresses two different genes: the first, light regulated, specific of green tissues and the second expressed in other tissues. In this work, we investigated whether in our clone, OsGog2 AC Y12595, this gene is up-regulated by light or it is expressed under darkness. Fd-Gogat specific activity, protein and mRNA increased after light treatment in rice shoots. In roots, the activity and the protein content remained constant, whereas the mRNA is repressed by light treatment. The results obtained using a specific probe, situated in the 3' untranslated region of the OsGog2 cDNA, indicated that OsGog2 gene is up-regulated by light and that its expression is tissue specific and suggested that a dark expressed Fd-Gogat gene could be present in rice similarly as in Arabidopsis.

Keywords: light regulation; nitrate assimilation; Oryza sativa; tissue specificity
Subjects: Arabidopsis thaliana; ferredoxin-dependent glutamate synthase (Fd-Gogat); nitrate assimilation; Northern blot analysis; Oryza sativa; rice; Southern blot analysis; Western blot analysis

Received: May 26, 2004; Accepted: September 27, 2005; Published: June 1, 2006  Show citation

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Mattana, M., Biazzi, E., Bertani, A., & Coraggio, I. (2006). Characterization of the Ferredoxin-Gogat gene (OsGog2 clone) expression in rice. Biologia plantarum, 50(2), 187-192. doi: 10.1007/s10535-006-0005-5
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