biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:198-204, 2006 | DOI: 10.1007/s10535-006-0007-3

Monitoring of cultivar identity in tissue culture-derived date palms using RAPD and AFLP analysis

M. M. Saker1,*, S. S. Adawy2, A. A. Mohamed2, H. A. El-Itriby2
1 Plant Biotechnology Department, National Research Center, Cairo, Egypt
2 Agriculture Genetic Engineering Research Institute, Agricultural Research Center, Cairo, Egypt

In the present study, two polymerase chain reaction (PCR)-based methods namely, randomly amplified polymophic DNA (RAPD) and amplification fragment length polymorphism (AFLP) were employed to assess genetic variations, which may appeared, in tissue culture-derived date palm (Phoenix dactylifera) offshoots. Analysis of RAPD banding patterns generated by PCR amplification using 37 random primers gave no evidences for somaclonal variations and the percentage of polymorphic bands in a total of 259 scored bands was zero. Meanwhile, analysis of AFLP banding patterns generated using 13 primer combinations pointed to minor genetic variations in the AFLP banding patterns. The percentage of genetic variations (polymorphism) in tissue culture-derived date palm offshoots belonging to cultivars Sakkoty, Gandila and Bertamoda was 2.6, 0.79 and 1 %, respectively, as revealed by AFLP analysis. The low percentage of genetic variations confirms the genetic stability of tissue culture-derived dry date palm cultivars.

Keywords: Phoenix dactylifera; micropropagation; cultivar identification; PCR
Subjects: cultivar and genotype differences; date palm; Phoenix dactylifera; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD); thidiazuron (TDZ)

Received: August 9, 2004; Accepted: April 17, 2005; Published: June 1, 2006  Show citation

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Saker, M.M., Adawy, S.S., Mohamed, A.A., & El-Itriby, H.A. (2006). Monitoring of cultivar identity in tissue culture-derived date palms using RAPD and AFLP analysis. Biologia plantarum, 50(2), 198-204. doi: 10.1007/s10535-006-0007-3
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    References

    1. Adawy, S., Hussein, E., El-Khishin, D., Saker, M., El-Itriby, H.: Genetic variability studies and molecular fingerprinting of some Egyptian date palm (Phoenix dactylifera L.) cultivars. II-RAPD and ISSR profiling.-Arab J. Biotechnol. 5: 225-236, 2002.
    2. Adawy, S.S., Hussein, E., Ebtissam. H.A., Ismail, S.E., El-Itriby, H.A.: Genomic diversity in date palm (Phoenix dactylifera L.) as revealed by AFLP in comparison to RAPDs and ISSRs-Arab J. Biotechnol. 8: 99-114, 2005.
    3. Al-Khayri, J., Al-Bahrany, A.: Growth, water content and proline accumulation in drought-stressed callus of date palm.-Biol. Plant. 48: 105-108, 2004. Go to original source...
    4. Bassam, J.B., Caetano-Anolles, G., Gresshoff, P.M.: Fast and sensitive silver staining of DNA in polyacrylamide gels.-Anal. Biochem. 196: 80-83, 1991. Go to original source...
    5. Bekheet, S.A., Taha, H.S., Saker, M.M.: Factors affecting in vitro multiplication of date palm.-Biol. Plant. 44: 431-433, 2001. Go to original source...
    6. Bekheet, S.A., Taha, H.S., Saker, M.M., Moursy, H.A.: A synthetic seed system of date palm through somatic embryogenesis encapsulation.-Ann. agr. Sci. Ain Shams Univ. (Cairo) 47: 325-337, 2002.
    7. Betal, S., Roy Chowdhury, P., Kundu, S., Sen Raychaudhuri, S.: Estimation of genetic variability of Vigna radiata cultivars by RAPD analysis.-Biol. Plant. 48: 205-209, 2004. Go to original source...
    8. Bhansali, R., Kaul, R., Dass, H.: Mass cloning of date palm plantlets through repetitive somatic embryogenesis.-J. Plant. Anat. Morphol. 5: 73-79, 1988.
    9. Chowdhury, U., Vasil, I.: Molecular analysis of plants regenerated from embryogenic cultures of hybrid sugar cane cultivars (Saccharum spp.).-Theor. appl. Genet. 86: 181-188, 1993. Go to original source...
    10. Dass, K., Kaul, R., Joshi, S., Bhansali, R.: In Vitro regeneration of date palm plantlets.-Curr. Sci. 58: 22-24, 1989.
    11. Gadalla, E.: Propagation of dry varieties of date palm.-Ph.D. Thesis. Faculty of Agriculture, Cairo University, Cairo 2003.
    12. Karp, A., Bright, J.: On the causes and origins of somaclonal variation.-Oxford Surv. Plant mol. cell Biol. 2: 199-234, 1985.
    13. McGreger, C., Lambert, C., Geryling, M., Louw, J., Warnich, L.: A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum) germplasm.-Euphytica 13: 135-144, 2000. Go to original source...
    14. Milbourne, D., Meyer, R.C., Bradshaw, J.E., Baird, E., Bonar, N., Provan, J., Powell, W., Waugh, R.: Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato.-Mol. Breed. 3: 127-136, 1997. Go to original source...
    15. Moursy, H.A., Saker, M.M.: Date palm problems and the need for biotechnology.-Bull. Fac. Agr. Univ. Cairo 49: 315-330, 1998.
    16. Mukherjee, A., Acharya, L., Mattagajasingh, I., Panda, P.: Molecular characterization of three Heritiera species using AFLP markers.-Biol. Plant. 47: 445-448, 2003/4. Go to original source...
    17. Murashige, T., Nakano T.: Tissue culture as a potential tool in obtaining polyploidy plants.-J. Heredity 57: 115-118, 1966. Go to original source...
    18. Porebski, S., Baley, L.G., Baum, B.R.: Modification of a CTAB DNA extraction protocol for plants containing high polysaccharides and polyphenol components.-Plant mol. Biol. Rep. 15: 8-15, 1997. Go to original source...
    19. Rival, A., Tregear, J., Verdeil, J.L., Richaud, F., Beule, T., Duval, Y., Hartmann, C.: Molecular search for mRNA and genomic markers of the oil palm "mantled" somaclonal variations.-Acta Hort. 461: 165-171, 1998. Go to original source...
    20. Russell, J.R. Fuller, J.D., Macaulay, M., Hatz, B.G., Jahoor, A., Powell, W., Waugh, R.: Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs, and RAPDs.-Theor appl. Genet. 86: 975-984, 1997. Go to original source...
    21. Saker, M., Bekheet, S., Taha, H., Fahmy, A., Moursy, H.: Detection of somaclonal variations in tissue culture-derived date palm plants using isozyme analysis and RAPD fingerprints.-Biol. Plant. 43: 347-351, 2000. Go to original source...
    22. Saker, M., Moursy, H., Bekheet, S.: In vitro propagation of Egyptian date palm: I-Morphogenic responses of immature embryos.-Bull. Fac. Agr. Univ. Cairo 49: 203-214, 1998.
    23. Saker, M.M., Kawshity, S., El-Bahr, M.K., Fahmy, A.: Flavonoid content and isozyme analysis of tissue culture-derived date palm clones.-Arab J. Biotechnol. 5: 207-216, 2002.
    24. Saker, M.M., Moursy, H.A.: Molecular characterization of Egyptian date palm: II-RAPD Fingerprints.-Arab J. Biotechnol. 2: 71-78, 1999.
    25. Salma, H., Saker, M.M.: DNA fingerprints of three different red palm weevil forms collected from infested Egyptian date palm orchards.-Arch. Phytopathol. Pflanz. 35: 1-8, 2002. Go to original source...
    26. Salman, M., Al-Jibouri, M., Al-Quadhy, K., Omar, S.: Isozyme and chromosomal analysis of tissue culture derived date palms.-Date Palm J. 6: 401-411, 1988.
    27. Sedra, M., Lashermes, P., Trouslot,, P., Comes, M.C., Haman, S.: Identification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markers.-Euphytica 103: 75-82, 1998. Go to original source...
    28. Sharma, S.K., Knox, M.R., Ellis, T.N.: AFLP analysis of the diversity and phylogeny of lens and its comparison with RAPD analysis.-Theor. appl. Genet. 93: 751-758, 1995. Go to original source...
    29. Singh, M., Saroop, J., Dhiman, B.: Detection of intra-clonal genetic variability in vegetatively propagated tea using RAPD markers.-Biol. Plant. 48: 113-115, 2004. Go to original source...
    30. Taylor, J., Fraser, A., Ko, L, Henry, J.: RAPD analysis of sugarcane during tissue culture.-In: Terzi, M., Cella, R., Falavigna, A. (ed.): Current Issues in Plant Molecular and Cellular Biology. Pp. 241-246. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    31. Tisserat, B.: Factors involved in the production of plantlets from date palm callus cultures.-Euphytica 31: 201-214, 1982. Go to original source...
    32. Virk, P.S., Zhu, J., Newbury, H., Bryan, G., Jackson, M., Ford-Lloyd, B.: Effectiveness of different classes of molecular markers for classifying and revealing variations in rice (Oryza sativa) germplasm.-Euphytica 112: 275-284, 2000. Go to original source...
    33. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M.: AFLP: a new technique for DNA fingerprinting.-Nucleic Acids Res. 23: 4407-4414, 1995. Go to original source...
    34. Williams, K., Kubelik, A., Livak, K., Rafalski, J., Tingey, V.: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.-Nucl. Acid Res 18: 6531-6535, 1990. Go to original source...
    35. Zaid, A., Hughes, H.: Water loss and polyethylene glycolmediated acclimatization of in vitro grown seedlings of 5 cultivars of date palm (Phoenix dactylifera L.) plantlets.-Plant Cell Rep. 14: 385-388, 1995. Go to original source...

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