biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:251-256, 2006 | DOI: 10.1007/s10535-006-0015-3

Protein composition of Cucurbita maxima and C. moschata seeds

H. A. Castro1, M. J. Galvez1, S. R. Gonzalez1, C. B. Villamil1,*
1 Departamento de Biologia, Bioquimica y Farmacia, Universidad Nacional del Sur, San Juan, Bahia Blanca, Argentina

Seeds of Cucurbita maxima, C. moschata and their interspecific hybrids were used to evaluate the intrapopulational and interpopulational variation of their protein composition. Three immunoprecipitating systems common to all the studied samples were detected by the Ouchterlony technique. Fourteen protein bands were identified by polyacrylamide gel electrophoresis (PAGE) whereas 23 bands were identified by sodiumdodecylsulfate (SDS)-PAGE. Using Western blotting (WB) also 23 bands were detected. The Jaccard's index of similarity calculated from SDS-PAGE and WB varied between 91 and 100 % for all the compared pairs of samples. These results demonstrate a high uniformity in the protein composition of all the samples and do not allow for their clear characterization.

Keywords: antigenic composition; polyacrylamide gel electrophoresis; Western blotting
Subjects: Cucurbita maxima; Cucurbita moschata; cultivar and genotype differences; proteins; Western blot analysis

Received: March 19, 2004; Accepted: June 7, 2005; Published: June 1, 2006  Show citation

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Castro, H.A., Galvez, M.J., Gonzalez, S.R., & Villamil, C.B. (2006). Protein composition of Cucurbita maxima and C. moschata seeds. Biologia plantarum, 50(2), 251-256. doi: 10.1007/s10535-006-0015-3
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