biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:265-271, 2006 | DOI: 10.1007/s10535-006-0017-1

A late blight resistant potato plant overexpresses a gene coding for α-galactosidase upon infection by Phytophthora infestans

D. Evers1,*, M. Ghislain2, L. Hoffmann1, J. F. Hausman1, J. Dommes3
1 CRP-Gabriel Lippmann, Cellule CREBS, Belvaux, Luxembourg
2 Department of Crop Improvement and Genetic Resources, International Potato Center, Lima, Peru
3 Department of Plant Biology B-22, University of Liege, Liege, Belgium

Late blight of potato, caused by Phytophthora infestans was studied by using a resistant clone of potato on one side and a susceptible clone on the other side. A gene coding putatively for an α-galactosidase has been isolated by mRNA reverse transcription polymerase chain reaction differential display and was shown to be differentially expressed between the resistant and the susceptible clone. α-Galactosidases catalyse the hydrolysis of α-1,6 linked α-galactose residues from oligosaccharides and it could be shown in the present work that raffinose content decreases at 30 h after infection by P. infestans in the resistant clone.

Keywords: raffinose; RT-PCR; Solanum phureja; Solanum tuberosum
Subjects: late blight of potato; Northern blot analysis; Phytophthora infestans; polymerase chain reaction (PCR); raffinose; reverse transcriptase polymerase chain reaction (RT-PCR); Solanum phureja; Solanum tuberosum; Southern blot analysis

Received: June 28, 2004; Accepted: June 7, 2005; Published: June 1, 2006  Show citation

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Evers, D., Ghislain, M., Hoffmann, L., Hausman, J.F., & Dommes, J. (2006). A late blight resistant potato plant overexpresses a gene coding for α-galactosidase upon infection by Phytophthora infestans. Biologia plantarum, 50(2), 265-271. doi: 10.1007/s10535-006-0017-1
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