biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:275-278, 2006 | DOI: 10.1007/s10535-006-0019-z

Stomatal characteristics during micropropagation of Wrightia tomentosa

P. Joshi1, N. Joshi1, S. D. Purohit1,*
1 Plant Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, India

A deviation from usually found characteristics of stomata in Wrightia tomentosa was noted during in vitro propagation. Increase in stomatal frequency in leaves of plants grown in vitro was observed with 29.4 % malformed stomata. The stomata were spherical, wide open, did not close in detached leaves even after 3 h. The leaves exhibited 93.4 % total water loss during 3-h period. Stomatal frequency, percentage of malformed stomata and rate of water loss declined in subsequent rooting phase. Nevertheless, for high survival rate plantlets were hardened under gradually decreasing air humidity either in partially opened glass bottles containing Soilrite™ moistened with 1/4 Murashige and Skoog nutrients or in pots covered with polyethylene bags. The stomatal characteristics of hardened plants were comparable to seedlings. Survival rate was more than 95 %.

Keywords: ex vitro transfer; hardening; in vitro culture; stomatal frequency; water relations
Subjects: auxins; cytokinins; ex vitro transfer; hardening; in vitro culture, micropropagation, propagation; stomatal density; water loss; Wrightia tomentosa

Received: March 3, 2004; Accepted: April 5, 2005; Published: June 1, 2006  Show citation

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Joshi, P., Joshi, N., & Purohit, S.D. (2006). Stomatal characteristics during micropropagation of Wrightia tomentosa. Biologia plantarum, 50(2), 275-278. doi: 10.1007/s10535-006-0019-z
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