biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:279-282, 2006 | DOI: 10.1007/s10535-006-0020-6

Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu

H. C. Wang1, J. T. Chen2,3,*, W. C. Chang1
1 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China
2 Institute of Biotechnology, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China
3 Department of Life Sciences, National University of Kaohsiung, Kaohsiung, Taiwan, Republic of China

Plant regeneration through somatic embryogenesis of Areca catechu L. was established using leaf, root and stem segments as explants. Embryogenic callus was induced and maintained on medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-2-methoxybenzoic acid (dicamba) at concentrations 2, 4, 6 and 8 mg dm-3 in darkness. Somatic embryos were found on primary callus in the presence of 2 and 4 mg dm-3 dicamba and during subculture on 2 - 8 mg dm-3 2,4-D or 2 - 4 mg dm-3 dicamba-containing media. Plantlet conversion from embryos was successfully achieved on growth regulator-free medium. The plants grew well when transplanted to containers in shaded greenhouse.

Keywords: betel nut; dicamba; 2,4-dichlorophenoxyacetic acid
Subjects: Areca catechu; betel nut; cytokinins; in vitro culture, callus growth and duration; in vitro culture, embryogenesis, somatic embryogenesis; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog

Received: April 21, 2004; Accepted: August 20, 2005; Published: June 1, 2006  Show citation

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Wang, H.C., Chen, J.T., & Chang, W.C. (2006). Somatic embryogenesis and plant regeneration from leaf, root and stem-derived callus cultures of Areca catechu. Biologia plantarum, 50(2), 279-282. doi: 10.1007/s10535-006-0020-6
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