biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:283-286, 2006 | DOI: 10.1007/s10535-006-0021-5

Influence of brassinosteroids on initiation of the root gravitropic response in Pisum sativum seedlings

G. N. Amzallag1,*, J. Vaisman1
1 The Judea Center for Research and Development, Carmel, Israel

In roots of Pisum sativum seedlings, the average lag-time required for initiation of the gravitropic response was reduced proportionally to the concentration of 24-epibrassinolide (EBL) added to the root solution (range of 10-13 to 10-8 M concentrations). A treatment with clotrimazole, a compound inhibiting steroid synthesis, prevents initiation of the gravitropic response. This effect was partly reverted by addition of EBL. From analysis of variability in the populations, it is suggested that BR conditions the root curvature through a gravitropic-induced change in sensitivity to the PGRs regulating cell elongation.

Keywords: clotrimazole; 24-epibrassinolide; pea
Subjects: brasinosteroids; clotrimazol; 24-epibrasinolide; gravitropic response of root; pea; Pisum sativum

Received: April 19, 2004; Accepted: September 5, 2005; Published: June 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Amzallag, G.N., & Vaisman, J. (2006). Influence of brassinosteroids on initiation of the root gravitropic response in Pisum sativum seedlings. Biologia plantarum, 50(2), 283-286. doi: 10.1007/s10535-006-0021-5
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Amzallag, G.N.: Data analysis in plant physiology: are we missing the reality?-Plant Cell Environ. 24: 881-890, 2001. Go to original source...
    2. Amzallag, G.N.: Brassinosteroids as metahormones: evidences from specific influence during critical period in Sorghum development.-Plant Biol. 4: 656-663, 2002. Go to original source...
    3. Ayub, M., Levell, M.J.: The inhibition of human prostatic aromatase activity by imidazole drugs including ketoconazole and 4-hydroxyandrostenedione.-Biochem. Pharmacol. 40: 1569-1575, 1990. Go to original source...
    4. Bach, T.J.: Selected natural and synthetic enzyme inhibitors of sterol biosynthesis as molecular probes for in vitro studies concerning the regulation of plant growth.-Plant Sci. 39: 183-187, 1985. Go to original source...
    5. Bishop, G.J., Koncz, C.: Brassinosteroids and plant steroid hormone signalling.-Plant Cell 2002(Suppl.): S97-S110, 2002. Go to original source...
    6. Choe, S., Dilkes, B.D., Fujioka, S., Takatsuto, S., Sakurai, A., Feldman, K.A.: The DWF4 gene of Arabidopsis encodes a cytochrome P450 that mediates multiple 22α-hydroxylation steps in brassinosteroid biosynthesis.-Plant Cell 10: 231-243, 1998. Go to original source...
    7. Chory, J., Li, J.: Gibberellins, brassinosteroids and light-regulated development.-Plant Cell Environ. 20: 801-806, 1997. Go to original source...
    8. Ephritikhine, G., Fellner, M., Vannini, C., Lapous, D., Barbier-Brygoo, H.: The sax1 dwarf mutant of Arabidopsis thaliana shows altered sensitivity of growth responses to abscisic acid, auxin, gibberellins and ethylene and is partially rescued by exogenous brassinosteroid.-Plant J. 18: 303-314, 1999. Go to original source...
    9. Evans, M.L., Ishikawa, H.: Cellular specificity of the gravitropic motor response in roots.-Planta 203(Suppl.): S115-S122, 1997. Go to original source...
    10. Gemzik, B., Parkinson, A.: Hydroxylation of 5α-androstane-3β,17β-diol by rat prostate microsome: potent inhibition by imidazole-type antimycotic drugs and lack of inhibition by steroid 5α-reductase inhibitor.-Arch. Biochem. Biophys. 296: 366-373, 1992. Go to original source...
    11. Gregory, L.E.: Acceleration of plant growth through seed treatment with brassins.-Amer. J. Bot. 68: 586-588, 1981. Go to original source...
    12. Halliday, K.J., Fankhauser, C.: Phytochrome-hormonal signalling networks.-New Phytol. 157: 449-463, 2003. Go to original source...
    13. Ishikawa, H., Hasenstein, K.H., Evans, M.L.: Computer-based video digitizer analysis of surface extension in maize roots. Kinetics of growth rate during gravitropism.-Planta 183: 381-390, 1991. Go to original source...
    14. Kauschmann, A., Jessop, A., Koncz, C., Szekeres, M., Willmitzer, L., Altmann, T.: Genetic evidence for an essential role of brassinosteroids in plant development.-Plant J. 9: 701-713, 1996. Go to original source...
    15. Kesy, J., Trzaskalska, E., Galoch, E., Kopcewicz, J. Inhibitory effect of brassinosteroids on the flowering of short-day plant Pharbitis nil.-Biol. Plant. 47: 597-600, 2003/4. Go to original source...
    16. Khripach, V.A., Zhabinskii, V.N., de Groot, A.E.: Brassinosteroids. A New Class of Plant Hormones.-Academic Press, San Diego, 1999.
    17. Kim, S.K., Chang, S.C., Lee, E.J., Chung, W.S., Kim, Y.S., Hwang, S., Lee, J.S.: Involvement of brassinosteroids in the gravitropic response of primary root of maize.-Plant Physiol. 123: 997-1004, 2000. Go to original source...
    18. Konings, H.: On the indoleacetic acid converting enzyme of pea roots and its relation to geotropism, straight growth and cell wall properties.-Acta bot. neerl. 13: 566-622, 1964. Go to original source...
    19. Krishna, P.: Brassinosteroid-mediated stress responses.-J. Plant Growth Regul. 22: 289-297, 2003. Go to original source...
    20. Meudt, W.T.: Investigations on mechanism of brassinosteroid response: VI. Effect of brassinolide on gravitropism of bean hypocotyls.-Plant Physiol. 83: 195-198, 1987. Go to original source...
    21. Nakajin, S., Takahashi, K., Shinoda, M.: Inhibitory effect of some imidazole antifungal compounds on the synthesis of 16-ene-C19-steroid catalyzed by pig testicular microsomes.-J. Steroid. Biochem. mol. Biol. 38: 95-99, 1991. Go to original source...
    22. Park, W.J.: Effect of epibrassinolide on hypocotyl growth of the tomato mutant diageotropica.-Planta 207: 120-124, 1998. Go to original source...
    23. Rorabaugh, P.A., Salisbury, F.B.: Gravitropism in higher plant shoots. VI. Changing sensitivity to auxin in gravistimulated hypocotyls.-Plant Physiol. 91: 1329-1338, 1989. Go to original source...
    24. Sasse, J.M.: Physiological actions of brassinosteroids: an update.-J. Plant Growth Regul. 22: 276-288, 2003. Go to original source...
    25. Still, D.W., Dahal, P., Bradford, K.J.: A single-seed assay for endo-β-mannanase activity from tomato endosperm and radicle tissues.-Plant Physiol. 113: 13-20, 1997. Go to original source...
    26. Suzuki, T., Miwa, K., Ishikawa, K., Yamada, H., Aiba, H., Mizuno, T.: The Arabidopsis sensor His-kinase, AHK4, can respond to cytokinins.-Plant Cell Physiol. 42: 107-113, 2001. Go to original source...
    27. Wolverton, C., Ishikawa, H., Evans, M.L.: The kinetics of root gravitropism: dual motors and sensors.-J. Plant Growth Regul. 21: 102-112, 2002. Go to original source...
    28. Zieschang, H.A., Sievers, A.: Graviresponse and the localization of its initiating cells in roots of Phleum pratense L.-Planta 184: 468-477, 1991. Go to original source...

    Return to the content