biologia plantarum

Biologia plantarum 50:291-294, 2006 | DOI: 10.1007/s10535-006-0023-3

Rapid clonal propagation of Vitex trifolia

L. V. Hiregoudar¹, H. N. Murthy^2,*, J. G. Bhat¹, A. Nayeem¹, B. P. Hema¹, E. J. Hahn², K. Y. Paek²

¹ Plant Tissue Culture Laboratory, Department of Botany, Karnatak University, Dharwad, Karnataka, India

² Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, South Korea

This report describes in vitro shoot induction and plant regeneration from mature nodal explants of Vitex trifolia L. on Murashige and Skoog (MS) medium fortified with benzylaminopurine (BAP), kinetin (KN), thidiazuron (TDZ), adenine (ADE), and 2-isopentenyladenine (2-iP) (0.25 - 10.0 μM). Multiple shoots differentiated directly without callus mediation within 3 weeks when explants were cultured on medium supplemented with cytokinins. The maximum number of shoots (9 shoots per explant) was developed on a medium supplemented with 5.0 μM BAP. Shoot cultures was established repeatedly subculturing the original nodal explant on the same medium. Rooting of shoots was achieved on half strength MS medium supplemented with 0.5 μM naphthaleneacetic acid (NAA). Rooted plantlets transferred to pots containing autoclaved soil and vermiculite mixture (1:1) showed 90 % survival when transferred to outdoor.

Keywords: auxins; cytokinins; in vitro culture; medicinal plant; micropropagation

Subjects: auxins; cytokinins; in vitro culture, micropropagation, propagation; medicinal plants; nutrient medium, Murashige and Skoog; Vitex trifolia

Received: May 21, 2004; Accepted: June 20, 2005; Published: June 1, 2006  Show citation