biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:295-299, 2006 | DOI: 10.1007/s10535-006-0024-2

Optimization of primer screening for evaluation of genetic relationship in rose cultivars

A. Mohapatra1, G. R. Rout1,*
1 Plant Biotechnology Division, Regional Plant Resource Centre, Bhubaneswar, Orissa, India

Optimization of primer screening for evaluation of genetic relationship in 34 cultivars of rose through random amplified polymorphic DNA (RAPD) markers was investigated. Four series of decamer primers were used for screening and optimization of RAPD analysis between which A and N series performed good amplification of fragments as compared with other series. The primers OPN-07 and OPN-15 produced maximum number of DNA fragments in Rosa hybrida cv. Anuraag. Some primer either did not produce amplification or produced very poor amplification. Further, ten selected primers were used for genetic analysis of 34 rose cultivars. The primer OPN-15 amplified 21 fragments in all cultivars tested. A total of 162 distinct DNA fragments (bands) ranging from 100 to 3400 base pairs were amplified by using 10 selected random primers. The cluster analysis indicated that these rose cultivars formed nine clusters.

Keywords: polymerase chain reaction; random amplified polymorphic DNA markers; Rosa hybrida
Subjects: cultivar and genotype differences; dendrogram, phylogenetic tree, phenogram; random amplified polymorphic DNA (RAPD); Rosa hybrida

Received: May 21, 2004; Accepted: January 12, 2005; Published: June 1, 2006  Show citation

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Mohapatra, A., & Rout, G.R. (2006). Optimization of primer screening for evaluation of genetic relationship in rose cultivars. Biologia plantarum, 50(2), 295-299. doi: 10.1007/s10535-006-0024-2
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