biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:303-306, 2006 | DOI: 10.1007/s10535-006-0026-0

Water relations, activities of antioxidants, ethylene evolution and membrane integrity of pigeonpea roots as affected by soil moisture

M. Jain1, A. S. Nandwal1,*, B. S. Kundu2, B. Kumar1, I. S. Sheoran1, N. Kumar1, A. Mann1, S. Kukreja1
1 Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar, Haryana, India
2 Department of Microbiology, CCS Haryana Agricultural University, Hisar, Haryana, India

The plants of pigeonpea (Cajanus cajan L.) cv. H77-216 were subjected to moderate [soil moisture content (SMC) = 7.3 ± 0.5 %] and severe (SMC = 4.3 ± 0.5 %) drought by withholding the irrigation at vegetative stage (45 d after sowing). The control plants were maintained at SMC of 11.0 ± 0.5 %. Half of the stressed plants were re-irrigated and their recovery was studied after 2 d. Leaf water potential, osmotic potential, and relative water content of leaf and root decreased significantly while a sharp rise in proline and total soluble sugars contents were noticed. Drought induced a significant increase in 1-aminocyclopropane 1-carboxylic acid (ACC) content and ACC oxidase activity which caused a considerable increase in ethylene evolution. Malondialdehyde content and relative stress injury were increased under drought whereas reverse was true for ascorbic acid content. The membrane integrity of roots decreased during stress and recovered on rehydration. The specific activity of total superoxide dismutase, ascorbate peroxidase, glutathione reductase, and glutathione transferase decreased to 37 - 78 %, 17 - 62 %, 29 - 36 % and 57 - 79 % at moderate and severe drought, respectively. The increase in activity of catalase and peroxidase could not overcome the accumulation of H2O2 content in the roots.

Keywords: ascorbic acid; lipid peroxidation; plant water status; proline; rehydration; total soluble sugars
Subjects: antioxidants, antioxidative enzymes; ascorbic acid, ascorbate; Cajanus cajan; catalase; glutathione reductase; glutathione transferase; lipid peroxidase, lipid peroxidation; malonyldialdehyde; pigeonpea; potentials, osmotic, water; proline; reactive oxygen species (ROS); relative water content (RWC); soil moisture, rehydration; sugars; superoxide dismutase (SOD); water stress

Received: May 17, 2004; Accepted: September 1, 2005; Published: June 1, 2006  Show citation

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Jain, M., Nandwal, A.S., Kundu, B.S., Kumar, B., Sheoran, I.S., Kumar, N., Mann, A., & Kukreja, S. (2006). Water relations, activities of antioxidants, ethylene evolution and membrane integrity of pigeonpea roots as affected by soil moisture. Biologia plantarum, 50(2), 303-306. doi: 10.1007/s10535-006-0026-0
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