biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:315-317, 2006 | DOI: 10.1007/s10535-006-0029-x

Peroxidase activity and lignification in soybean root growth-inhibition by juglone

P. A. F. Bohm1, F. M. L. Zanardo1, M. L. L. Ferrarese1, O. Ferrarese-Filho1,*
1 Department of Biochemistry, University of Maringa, Maringa, PR, Brazil

The changes in activities of soluble and cell wall-bound peroxidases and lignin contents in juglone-stressed soybean (Glycine max) seedlings and their relationships with root growth were investigated. Soybean seedlings (3-d-old) were cultivated in nutrient solution supplemented with 0.5 to 25 μM juglone for 24 h. Length and dry mass of roots decreased after 5 to 25 μM juglone treatments. Low juglone concentrations (≤ 1 μM) increased soluble peroxidase activity, while high concentrations (≥ 10 μM) inhibited activities of soluble and cell wall-bound peroxidases. Juglone (≤ 1 μM) did not affect lignin content but highly increased lignification after 5 to 25 μM treatments. Results indicate that lignification may be an important step in root growth reduction of juglone-stressed soybean.

Keywords: allelochemical; allelopathy; Glycine max; lignin; naphthoquinone
Subjects: allelopathy; Glycine max; growth analysis, plant development, biomass and yield enhancement; lignification, lignin; peroxidase; root, rooting; soybean

Received: August 23, 2004; Accepted: September 13, 2005; Published: June 1, 2006  Show citation

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Bohm, P.A.F., Zanardo, F.M.L., Ferrarese, M.L.L., & Ferrarese-Filho, O. (2006). Peroxidase activity and lignification in soybean root growth-inhibition by juglone. Biologia plantarum, 50(2), 315-317. doi: 10.1007/s10535-006-0029-x
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