biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:321-325, 2006 | DOI: 10.1007/s10535-006-0044-y

Histological characterization of in vitro adventitious organogenesis in Citrus sinensis

W. A. B. de Almeida1, F. de A. A. Mourao Filho2, B. M. J. Mendes3, A. P. M. Rodriguez3,*
1 Departamento de Fitotecnia, Faculdade de Agronomia, UFBA, Cruz das Almas, BA, Brazil
2 Departamento de Producao Vegetal, ESALQ, Universidade de Sao Paulo, Piracicaba, SP, Brazil
3 Laboratorio de Biotecnologia Vegetal, CENA, Universidade de Sao Paulo, Piracicaba, SP, Brazil

The adventitious bud development was induced in epicotyl segments of Valencia sweet orange (Citrus sinensis L. Osbeck). Seeds were cultured in vitro for three weeks in the dark, followed by one week at a 16-h photoperiod. Epicotyl segments were cultured horizontally for the induction of organogenesis in Murashige and Tucker (1969, MT) culture medium supplemented with 1.0 mg dm-3 benzylaminopurine. Samples were observed by light and scanning electron microscopy from day zero to day 25, when buds were well grown. It was shown that the adventitious buds originated directly from the cambial region on the cut ends of the explants.

Keywords: adventitious shoots; benzylaminopurine; cambium; direct organogenesis; epicotyl; ontogenesis; Valencia sweet orange
Subjects: cambium; Citrus sinensis; cytokinins; epicotyl; in vitro culture, organogenesis; nutrient medium, Murashige and Tucker; ontogeny, plant; sweet orange

Received: November 18, 2004; Accepted: April 18, 2005; Published: September 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
de Almeida, W.A.B., de Mourao Filho, F.A.A., Mendes, B.M.J., & Rodriguez, A.P.M. (2006). Histological characterization of in vitro adventitious organogenesis in Citrus sinensis. Biologia plantarum, 50(3), 321-325. doi: 10.1007/s10535-006-0044-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Almeida, W.A.B. de, Mourao Filho, F. de A.A., Mendes, B.M.J., Rodriguez, A.P.M.: In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia.-Sci. Agr. 59: 35-40, 2002. Go to original source...
    2. Almeida, W.A.B. de, Mourao Filho, F. de A.A., Pino, L.E., Boscariol, R.L., Rodriguez, A.P.M., Mendes, B.M.J.: Genetic transformation and plant recovery from mature tissues of Citrus sinensis L. Osbeck.-Plant Sci. 164: 203-211, 2003a. Go to original source...
    3. Almeida, W.A.B. de, Mourao Filho, F. de A.A., Mendes, B.M.J., Pavan, A., Rodriguez, A.P.M.: Agrobacterium-mediated transformation of Citrus sinensis and C. limonia epicotyl segments.-Sci. Agr. 60: 23-29, 2003b. Go to original source...
    4. Cervera, M., Pina, J.A., Juarez, J., Navarro, L., Pena, L.: Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration.-Plant Cell Rep. 18: 271-278, 1998. Go to original source...
    5. Garcia-Luis, A., Bordon, Y., Moreira-Dias, J.M., Molina, R.V., Guardiola, J.L.: Explant orientation and polarity determine the morphogenic response of epicotyl segments of Troyer citrange.-Ann. Bot. 84: 715-723, 1999. Go to original source...
    6. Gosal, S.S., Gill, M.I.S., Grewal, H.S.: Somatic embryogenesis in Citrus species.-In: Jain, S., Gupta, P., Newton, R. (ed.): Somatic Embryogenesis in Woody Plants. Pp. 1-21. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
    7. Grosser, J.W., Ollitrault, P., Olivares-Fuster, O.: Somatic hybridization in citrus: an effective tool to facilitate variety improvement.-In Vitro cell. dev. Biol. Plant 36: 434-449, 2000. Go to original source...
    8. Hassanein, A.M., Azooz, M.M.: Propagation of Citrus reticulata via in vitro seed germination and shoot cuttings.-Biol. Plant. 47: 173-177, 2003/4. Go to original source...
    9. Hiregoudar, L.V., Ashok Kumar, H.G., Murthy, H.N.: In vitro culture of Feronia limonia (L.) Swingle from hypocotyls and internodal explants.-Biol. Plant. 49: 41-45, 2005. Go to original source...
    10. Murashige, T., Tucker, D.P.H.: Growth factor requirement of Citrus tissue culture.-In: Chapman, H.D. (ed.): Proceedings of the First International Citrus Symposium. Pp. 1155-1161. University of California, Riverside 1969.
    11. Pena, L., Cervera, M., Juarez, J., Navarro, A., Pina, J.A., Duran-Vila, N., Navarro, L.: High efficiency Agrobacterium-mediated transformation and regeneration of Citrus.-Plant Sci. 104: 183-191, 1995. Go to original source...
    12. Rodriguez, A.P.M., Wetzstein, H.Y.: A morphological and histological comparison of the initiation and development of pecan (Carya illinoinensis) somatic embryogenic cultures induced with naphthaleneacetic acid or 2,4-dichlorophenoxyacetic acid.-Protoplasma 204: 71-83, 1998. Go to original source...

    Return to the content