biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:421-424, 2006 | DOI: 10.1007/s10535-006-0060-y

Variability for the in vitro culture response in tomato recombinant inbred lines

G. R. Pratta1,2, R. Zorzoli2,*, L. A. Picardi2, E. M. Valle1
1 Fac. Cs. Bioq. Farm., UNR, IBR - CONICET, Rosario, Argentina
2 Catedra de Genetica, Facultad de Ciencias Agrarias UNR, CIUNR, Zavalla, Argentina

The aim of this work was to estimate genetic variability for in vitro culture response of recombinant inbred lines (RILs) of the genus Lycopersicon. The callus percentage (C), the regeneration percentage (R) and the productivity rate (PR) were evaluated 45 d after culture initiation in a set of 16 elite tomato RILs and their parents. The narrow sense heritability (h2) values were 0.38 ± 0.04 for C, 0.46 ± 0.04 for R, and 0.28 ± 0.03 for R, while the genetic correlation (r g ) values were -0.96 ± 0.07 between C and R, 0.81 ± 0.14 between PR and R, and -0.79 ± 0.16 between PR and C. Three AFLP markers associated to the in vitro traits were identified.

Keywords: amplified fragment length polymorphism (AFLP); callus production; Lycopersicon esculentum; Lycopersicon pimpinellifolium; plant breeding; shoot formation
Subjects: genetic diversity, variability; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; Lycopersicon esculentum; Lycopersicon pimpinellifolium

Received: August 24, 2004; Accepted: July 2, 2005; Published: September 1, 2006  Show citation

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Pratta, G.R., Zorzoli, R., Picardi, L.A., & Valle, E.M. (2006). Variability for the in vitro culture response in tomato recombinant inbred lines. Biologia plantarum, 50(3), 421-424. doi: 10.1007/s10535-006-0060-y
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