biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:441-443, 2006 | DOI: 10.1007/s10535-006-0065-6

In vitro micropropagation of Ruscus aculeatus

E. Moyano1, M. Montero2, M. Bonfill2, R. M. Cusido2, J. Palazon2,*, M. T. Pinol2
1 Departament de Ciencies Experimental i de la Salut, Universitat Pompeu Fabra, Barcelona, Spain
2 Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Barcelona, Barcelona, Spain

We have developed three protocols for the rapid micropropagation of Ruscus aculeatus. The primary explants utilised were immature embryos, aerial buds excised from rhizomes and shoot buds regenerated from organogenic calli. In order to increase the plant regeneration from the primary explants, we used organogenic calli from cladode, stem and rhizome segments. We tested more than 20 culture media for callus induction and shoot regeneration and the best results were obtained when rhizome segments were cultured on Murashige and Skoog medium supplemented with 0.5 mg dm-3 2,4-dichlorophenoxyacetic acid and 1 mg dm-3 kinetin.

Keywords: 2,4-dichlorophenoxyacetic acid; embryo cultures; kinetin; naphtalenacetic acid; organogenic callus
Subjects: auxins; cytokinins; in vitro culture, organogenesis; Ruscus aculeatus

Received: June 7, 2004; Accepted: May 2, 2005; Published: September 1, 2006  Show citation

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Moyano, E., Montero, M., Bonfill, M., Cusido, R.M., Palazon, J., & Pinol, M.T. (2006). In vitro micropropagation of Ruscus aculeatus. Biologia plantarum, 50(3), 441-443. doi: 10.1007/s10535-006-0065-6
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