biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:457-460, 2006 | DOI: 10.1007/s10535-006-0070-9

A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels

A. Krahulcova1,*, J. Suda1,2
1 Institute of Botany, Academy of Sciences of the Czech Republic, Pruhonice, Czech Republic
2 Department of Botany, Charles University, Prague, Czech Republic

Flow cytometric analysis of ten bulked seeds is proposed to quantify particular embryo ploidy classes in Hieracium. The method is recommended 1) for the detection and quantification of residual sexuality in facultative apomicts, which can generate progeny from heteroploid crosses, 2) for the quantitative screening of pollen donors with different ploidy levels, based on the fertilization success of the maternal plant, and 3) for the screening of parents producing a high proportion of polyhaploids.

Keywords: facultative apomixis; polyhaploids; reproduction routes; seed progeny screening
Subjects: Hieratium rubrum; ploidy; seed

Received: October 29, 2004; Accepted: May 10, 2005; Published: September 1, 2006  Show citation

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Krahulcova, A., & Suda, J. (2006). A modified method of flow cytometric seed screen simplifies the quantification of progeny classes with different ploidy levels. Biologia plantarum, 50(3), 457-460. doi: 10.1007/s10535-006-0070-9
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