biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:465-468, 2006 | DOI: 10.1007/s10535-006-0072-7

Electric current affects the rate of development in isolated apical parts of rape in vitro

M. Filek1, J. Biesaga-Koścelniak1, I. Marcińska1, J. Krekule2,*, I. Macháčkova2, F. Dubert1
1 Laboratory of Plant Growth and Development, Polish Academy of Sciences, Krakow, Poland
2 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Praha, Czech Republic

Apical parts of stems of Brassica napus L. var. oleifera cv. Gorczanski (winter rape) and cv. Mlochowski (spring rape), grown in vitro, were subjected to direct electric current (DC) of different polarity, duration and voltage. The positive orientation of DC, i.e. anode attached to the apical part and cathode to the medium, markedly enhanced the differentiation of the apical meristem in winter rape. The reverse polarity was without effect. DC treatment of positive polarity resulted in spring rape in transition of all explants to generative state while 70 % of non-treated plants remained at vegetative stage. Even negative orientation of DC brought about a rise in percentage of flowering plants with regard to control. The developmental effects of DC were dependent only to a low degree or not at all on duration and voltage of the treatment.

Keywords: apical meristem differentiation; flowering factors; vernalization
Subjects: Brassica napus; cytokinins; flowering; growth analysis, plant development, biomass and yield enhancement; in vitro culture, regeneration, proliferation, differentiation; vernalisation

Received: November 30, 2004; Accepted: April 21, 2005; Published: September 1, 2006  Show citation

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Filek, M., Biesaga-Koścelniak, J., Marcińska, I., Krekule, J., Macháčkova, I., & Dubert, F. (2006). Electric current affects the rate of development in isolated apical parts of rape in vitro. Biologia plantarum, 50(3), 465-468. doi: 10.1007/s10535-006-0072-7
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