biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:507-513, 2006 | DOI: 10.1007/s10535-006-0080-7

In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants

M. R. Rady1,*
1 Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki Giza, Cairo, Egypt

A protocol is established for regeneration of the economically important cut flower plant, Gypsophila paniculata L., using shoot tips explants. Multiple shoots were obtained on Murashige and Skoog medium fortified with 0.5 mg dm-3 each of α-naphthaleneacetic acid and 6-benzyladenine. Addition of 10 g dm-3 agar promoted shoot proliferation and reduced the degree of shoot vitrification. Transfer to 3 mg dm-3 indole-3-butyric acid containing medium produced optimum root initiation and development. The produced plants as well as intact plants were subjected to the random amplified polymorphic DNA (RAPD) analysis. Using 9 primers, the total number of amplification products generated by polymerase chain reaction was 142 bands (15.7 bands per primer), of which 7.74 % showed polymorphism. The analysis of bands recorded, showed 92.25 % similarity. The results indicated that very low variation at the DNA level occurred during in vitro culture of Gypsophila.

Keywords: auxins; cut flower plant; cytokinins; micropropagation; molecular marker; polymerase chain reaction
Subjects: auxins; cytokinins; Gypsophilla paniculata; in vitro culture, agar, alginate; in vitro culture, micropropagation, propagation; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Murashige and Skoog; polymerase chain reaction (PCR); random amplified polymorphic DNA (RAPD)

Received: November 1, 2004; Accepted: September 21, 2005; Published: December 1, 2006  Show citation

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Rady, M.R. (2006). In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants. Biologia plantarum, 50(4), 507-513. doi: 10.1007/s10535-006-0080-7
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