biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 50:559-564, 2006 | DOI: 10.1007/s10535-006-0088-z

Changes in nonpolar aldehydes in bean cotyledons during ageing

N. Wilhelmová1,*, P. M. D. N. Domingues2, M. Srbová3, H. Fuksová3, J. Wilhelm3
1 Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic
2 Departamento de Química, Campus Universitário de Santiago, Aveiro, Portugal
3 2nd Medical Faculty, Charles University, Prague, Czech Republic

Ageing of plant organs is accompanied by an increased production of free radicals what results in membrane lipid peroxidation. Non-polar aldehydes originating from this process interact with the cellular material to form the fluorescent end-products, lipofuscin-like pigments (LFP). Their formation was studied both qualitatively and quantitatively in ageing of bean cotyledons. The concentration of lipofuscin-like pigments increased 9-fold in 14-d-old (senescent) cotyledons in relation to 8-d-old (young) cotyledons. HPLC fractionation patterns indicate changes in their composition during ageing. The LFP increase in old cotyledons was accompanied by elevated levels of non-polar aldehydes that increased during ageing to 167 %. The composition of aldehydes was studied by mass spectrometry. The most abundant fraction in both young and old cotyledon was represented by C12 aldehydes, which comprised both saturated and unsaturated species. We have observed differences in abundances of individual aldehydes between the young and the old cotyledons that might explain the differences in the composition of lipofuscin-like pigments. These results support the involvement of free radicals in plant ageing; however, it is suggested that plant aldehydic products of lipid peroxidation differ from those found in animals.

Keywords: lipid peroxidation; lipofuscin-like pigments (LFP); mass spectrometry; Phaseolus vulgaris
Subjects: bean; cotyledon; in vitro culture, callus growth and duration; lipid peroxidase, lipid peroxidation; lipofuscin-like pigments; nonpolar aldehydes; Phaseolus vulgaris

Received: February 2, 2005; Accepted: June 6, 2005; Published: December 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wilhelmová, N., Domingues, P.M.D.N., Srbová, M., Fuksová, H., & Wilhelm, J. (2006). Changes in nonpolar aldehydes in bean cotyledons during ageing. Biologia plantarum, 50(4), 559-564. doi: 10.1007/s10535-006-0088-z
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Beckman, J.K., Morley, S.A., Jr., Greene, H.L.: Analysis of aldehydic lipid peroxidation products by TLC/densitometry.-Lipids 26: 155-161, 1991. Go to original source...
    2. Bolgar, M.S., Gaskell, S.J.: Determination of the sites of 4-hydroxy-2-nonenal adduction to protein by electrospray tandem mass spectrometry.-Anal. Chem. 68: 2325-2330, 1996. Go to original source...
    3. Chio, S., Tappel, A.L.: Synthesis and characterization of the fluorescent products.-Biochemistry 8: 2821-2827, 1969. Go to original source...
    4. Chowdhury, P.K., Halder, M., Choudhury, P.K., Kraus, G.A., Desai, M.J., Armstrong, D.W., Casey, T.A., Rasmussen, M.A., Petrich, J.W.: Generation of fluorescent adduts of malondialdehyde and amino acids: Toward an understanding of lipofuscin.-Photochem. Photobiol. 79: 21-25, 2004. Go to original source...
    5. Dhindsa, R.J., Dhindsa, P.P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 32: 93-101, 1981. Go to original source...
    6. Esterbauer, H., Koller, E., Slee, R.G., Koster, J.F.: Possible involvement of the lipid-peroxidation product 4-hydroxynonenal in the formation of fluorescent chromolipids.-Biochem. J. 239: 405-409, 1986. Go to original source...
    7. Gerstbrein, B., Stamatas, G., Kollias, N., Driscoll, M.: In vivo spectrofluorimetry reveals endogenous biomarkers that report healthspan and dietary restriction in Caenorhabditis elegans.-Aging Cell 4: 127-137, 2005. Go to original source...
    8. Hudak, K., Yao, K., Thompson, J.E.: Release of fluorescent peroxidized lipids from membranes in senescing tissue by blebbing of lipid-protein particles.-Hort. Sci. 30: 209-213, 1995. Go to original source...
    9. Kikugawa, K., Machida, Y., Kida, M., Kurechi, T.: Studies on peroxidized lipid. III. Fluorescent pigments derived from the reaction of malonaldehyde and amino acids.-Chem. Pharm. Bull. 29: 3003-3011, 1981. Go to original source...
    10. Maguire, Y.P., Haard, N.F.: Fluorescent product accumulation in ripening fruit.-Nature 258: 599-600, 1975. Go to original source...
    11. McLafferty, F.W., Stauffer, D.A., Loh, S.Y., Wesdemiotis, C.: Unknown identification using reference mass spectra. Quality evaluation databases.-J. amer. Soc. Mass Spectrometry 10: 1229-1240, 1999. Go to original source...
    12. Merzlyak, M.N., Plakunova, O.V., Gostimsky, S.A., Rumyantseva, V.B., Novak, K.: Lipid peroxidation in and photo-damage to a light-sensitive chlorescence pea mutant.-Physiol. Plant. 62: 329-334, 1984. Go to original source...
    13. Pauls, K.P., Thompson, J.E.: Evidence for the accumulation of peroxidized lipids in membranes of senescing cotyledons.-Plant Physiol. 75: 1152-1157, 1984. Go to original source...
    14. Procházková, D., Wilhelmová, N.: Changes in antioxidative protection in bean cotyledons during natural and continuous irradiation-accelerated senescence.-Biol. Plant. 48: 33-39, 2004. Go to original source...
    15. Sohal, R.S., Allen, R.G.: Relationship between oxygen metabolism, aging and development.-In: Sohal, R.S. (ed.): Advances in Free Radical Biology and Medicine.Vol. 2. Pp. 117-159. Pergamon Press, New York 1986. Go to original source...
    16. Tamura, H., Shibamoto, T.: Gas chromatographic analysis of malonaldehyde and 4-hydroxy-2-(E)-nonenal produced from arachidonic acid and linoleic acid in a lipid peroxidation model system.-Lipids 26: 170-173, 1991. Go to original source...
    17. Wang, W., Sun, G.-C., Lin, Z.-F.: Lipofuscin-like pigments in chloroplasts and effects of malonaldehyde and alpha-tocopherol on its accumulation.-Plant. Physiol. Commun. 3: 31-34, 1988.
    18. Wilhelm, J., Wilhelmová, N.: Accumulation of lipofuscin-like pigments in chloroplasts from senescent leaves of Phaseolus vulgaris.-Photosynthetica 15: 55-60, 1981.
    19. Wilhelmová, N., Procházková, D., Macháĉková, I., Vágner, M., Srbová, M., Wilhelm, J.: The role of cytokinins and ethylene in bean cotyledon senescence. The effect of free radicals.-Biol. Plant. 48: 523-529, 2004. Go to original source...
    20. Wilhelmová, N., Wilhelm, J., Kutík, J., Haisel, D.: Changes in French bean cotyledon composition associated with modulated life-span.-Photosynthetica 34: 377-391, 1997. Go to original source...
    21. Yao, K., Paliath, G., Thompson, J.E.: Localized of peroxidized lipids in nonsedimentable microvesicles of senescing bran cotyledons.-J. exp. Bot. 44: 1267-1274, 1993. Go to original source...
    22. Yin, D.: Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores.-Free Radical Biol. Med. 21: 871-888, 1996. Go to original source...

    Return to the content