biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 50:574-580, 2006 | DOI: 10.1007/s10535-006-0090-5

Polyamine contents, ethylene synthesis, and BrACO2 expression during turnip germination

M. I. Puga-Hermida1, M. Gallardo1, M. C. Rodriguez-Gacio2, A. J. Matilla3,*
1 Lab. Fisiología Vegetal, Facultade de Biología, Edificio de Ciencias Experimentales, Universidad de Vigo, Vigo, Spain
2 Lab. Fisiología Vegetal, Escuela Politécnica Superior, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
3 Lab. Fisiología Vegetal, Facultade de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, Spain

Contents of total free [PA(S)] and conjugated polyamines [PA(SH), PA(PH)] were higher in turnip (Brassica rapa L. cv. Rapa) seeds during imbibition (0-36 h) and radicle protrusion (36-48 h) than during the further growth (10 d). Ethylene production was activated with the protrusion, reaching a maximum at the second day of germination and dropping afterwards. The application of ethrel accelerated radicle emergence but the direct intervention of ethylene in the breaking of the seed coat was not clear from the use of ethylene-biosynthesis inhibitors (CoCl2 and AVG). Finally, in this work the gene BrACO2 was characterized. Although its expression was not detected in seeds through zygotic embryogenesis, it increased concomitantly with the germination process.

Keywords: Brassica rapa; Cruciferae; conjugated polyamines; ethrel; free polyamines; seed
Subjects: 1-aminocyclopropane-1-carboxylateoxidase; Brassica rapa; cadaverine; dendrogram, phylogenetic tree, phenogram; ethrel-2-chloroethylphosphonic acid; ethylene synthesis; gibberellic acid, gibberellins; Northern blot analysis; polyamines; putrescine; seed; spermidine; spermine; turnip

Received: November 10, 2004; Accepted: September 12, 2005; Published: December 1, 2006  Show citation

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Puga-Hermida, M.I., Gallardo, M., Rodriguez-Gacio, M.C., & Matilla, A.J. (2006). Polyamine contents, ethylene synthesis, and BrACO2 expression during turnip germination. Biologia plantarum, 50(4), 574-580. doi: 10.1007/s10535-006-0090-5
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