biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:617-623, 2006 | DOI: 10.1007/s10535-006-0097-y

Short-term effect of elevated CO2 concentration and high irradiance on the antioxidant enzymes in bean plants

M. Lambreva1,*, K. Christov1, T. Tsonev1
1 Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

The effect of short-term exposure to elevated CO2 concentration and high irradiance on the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), guaiacol peroxidases (GPX) and catalase (CAT), and on the extent of the lipid peroxidation was studied in bean (Phaseolus vulgaris L.) plants. Plants were exposed for 4 d (8 h a day) to irradiance of 100 (LI) or 1000 (HI) μmol m-2 s-1 at ambient (CA, 350 μmol mol-1) or elevated (CE, 1300 μmol mol-1) CO2 concentration. Four-day exposure to CE increased the leaf dry mass in HI plants and RuBPC activity and chlorophyll content in LI plants. Total soluble protein content, leaf dry matter and RuBPC activity were higher in HI than in LI plants, although the HI and CE increased the contents of malonyldialdehyde and H2O2. Under CA, exposure to HI increased the activity of APX and decreased the total SOD activity. Under CE, HI treatment also activated APX and led to reduction of both, SOD and GPX, enzymes activities. CE considerably reduced the CAT activity at both irradiances, possibly due to suppressed rate of photorespiration under CE conditions.

Keywords: ascorbate peroxidase; catalase; guaiacol peroxidase; lipid peroxidation; Phaseolus vulgaris; RuBPC; superoxide dismutase
Subjects: antioxidants, antioxidative enzymes; ascorbate peroxidase; catalase; CO2 concentration, elevated, enrichment; guaiacol peroxidase; lipid peroxidase, lipid peroxidation; malonyldialdehyde; Phaseolus vulgaris; (photosynthetic) photon flux density; photosystem 2; ribulose-1,5-bisphosphate carboxylase/oxygenase; superoxide dismutase (SOD)

Received: November 30, 2004; Accepted: May 31, 2005; Published: December 1, 2006  Show citation

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Lambreva, M., Christov, K., & Tsonev, T. (2006). Short-term effect of elevated CO2 concentration and high irradiance on the antioxidant enzymes in bean plants. Biologia plantarum, 50(4), 617-623. doi: 10.1007/s10535-006-0097-y
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