biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:624-634, 2006 | DOI: 10.1007/s10535-006-0098-x

Inhibition of photosynthetic processes in foliose lichens induced by temperature and osmotic stress

J. Hájek1,*, M. Barták1, J. Dubová1
1 Department of Plant Physiology and Anatomy, Faculty of Science, Masaryk University, Brno, Czech Republic

Negative effects of osmotically-induced dehydration of two foliose lichen species, Lasallia pustulata and Umbilicaria hirsuta, was studied at physiological (22 °C), low (5 °C) and freezing temperature (-10 °C), using chlorophyll (Chl) fluorescence. In both species, exposure to increasing sucrose concentrations led to a pronounced decrease in potential (FV/FM), and actual (Φ2) quantum yields of photochemical processes in photosystem 2. L. pustulata was more sensitive to osmotic stress, because comparable osmotic dehydration inhibited FV/FM and Φ2 more than in U. hirsuta. Critical concentration of sucrose that fully inhibited photochemical processes of photosynthesis was 2.5 M, which represented water potential (Ψw) of -18.8 MPa. Decrease in background Chl fluorescence (F0) and increase in non-photochemical quenching (qN) revealed two phases of osmotic stress in lichens: phase I with no change (Ψw 0 to -6.6 MPa) and phase II (Ψw -11.3 to -18.8 MPa) typical by substantial change in Chl fluorescence parameters. Effects of thallus anatomy on species-specific response to osmotic dehydration is discussed and attributed to the results obtained by optical microscopy and Chl fluorescence imaging technique.

Keywords: photosynthesis; water potential; dehydration; Lasallia pustulata; Umbilicaria hirsuta; sucrose; chlorophyll fluorescence; thallus anatomy
Subjects: chlorophyll fluorescence; Lasallia oustulata; lichen; osmotic stress; potentials, osmotic, water; quantum yield; sucrose; sugars; temperature, photosynthetic processes; thallus, lichen anatomy; Umbilicaria hirsuta

Received: December 15, 2004; Accepted: October 17, 2005; Published: December 1, 2006  Show citation

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Hájek, J., Barták, M., & Dubová, J. (2006). Inhibition of photosynthetic processes in foliose lichens induced by temperature and osmotic stress. Biologia plantarum, 50(4), 624-634. doi: 10.1007/s10535-006-0098-x
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