biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:660-666, 2006 | DOI: 10.1007/s10535-006-0103-4

Variability in sequence of Strawberry vein banding virus

D. Hanzliková Vašková*1,*, J. Špak1, K. Petrzik1
1 Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic

The variability of the Strawberry vein banding virus (SVBV) isolates was investigated. In total 267 strawberry plants from 6 European countries and North America were tested for the presence of SVBV. Only 4 plants were positive. Partial genomic sequences of the capsid protein gene of three North American SVBV isolates were determined. Only minor sequence variability (0.7 %) was observed during a comparison with existing nucleotide data of the European and the North American isolates (9 isolates). No variability at all could be found in the annealing regions of primers and probes used for molecular detection of SVBV for these isolates. However, a comparison to a sequence of a Chinese isolate published recently revealed a much higher DNA sequence difference (9.5 %) of this isolate.

Keywords: caulimovirus; diversity; Fragaria sp; NASBA; PCR; quarantine
Subjects: caulimovirus; Fragaria sp.; polymerase chain reaction (PCR); quarantine; Strawberry vein banding virus

Received: September 21, 2004; Accepted: May 24, 2005; Published: December 1, 2006  Show citation

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Hanzliková Vašková, D., Špak, J., & Petrzik, K. (2006). Variability in sequence of Strawberry vein banding virus. Biologia plantarum50(4), 660-666. doi: 10.1007/s10535-006-0103-4
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